| HPLC法测定盐酸多沙普仑葡萄糖注射液的含量 |
| 投稿时间：2012-04-06 修订日期：2012-05-17 点此下载全文 |
| 引用本文：薛志红,王盛豪,潘世茹.HPLC法测定盐酸多沙普仑葡萄糖注射液的含量[J].药学实践杂志,2013,31(1):64~66 |
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| 基金项目:安徽省高等学校省级教学质量与教学改革工程项目(20101142). |
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| 中文摘要:目的 建立盐酸多沙普仑葡萄糖注射液的高效液相色谱含量测定方法。方法 采用COSMOSIL C18(150 mm×4.6 mm,5 μm)色谱柱,以0.82 μg/L醋酸钠溶液(用冰醋酸调pH值为4.5) -乙腈=70:30为流动相,检测波长为214 nm。结果 盐酸多沙普仑浓度在13~91 μg/ml范围内呈良好的线性关系(r=0.999 8)。平均加样回收率为99.3%,RSD为0.81%(n=9)。结论 此方法简便、准确、灵敏度高、专属性强。 |
| 中文关键词:高效液相色谱法 盐酸多沙普仑 含量测定 |
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| Determination of doxapram hydrochloride glucose injection by HPLC |
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| Abstract:Objective To establish an HPLC method for determination of doxapram hydrochloride glucose injection.Methods COSMOSIL C18 (150 mm×4.6 mm,5 μm) column was adopted. The mobile phase consisted of 0.82 μg/L CH3COONa (using Glacial acetic acid adjust to pH 4.5)-acetonitrile (70:30). The detection wavelength was 214 nm.Results The linearity of doxapram hydrochloride concentration was in the range of 13~91 μg/ml (r=0.999 8). The average recovery was 99.3％,RSD=0.81% (n=9). Conclusion The method was simpleaccurate and sensitive with high specificity. |
| keywords:HPLC doxapram hydrochloride determination |
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