| HPLC测定鼠曲草中槲皮素的含量 |
| 投稿时间：2012-02-14 修订日期：2012-04-24 点此下载全文 |
| 引用本文：韦少宣,夏爱军,廖厚知.HPLC测定鼠曲草中槲皮素的含量[J].药学实践杂志,2012,30(6):451~453 |
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| 中文摘要:目的 建立鼠曲草中槲皮素的含量测定方法。方法 采用VP-ODS C18(4.6 mm×150 mm, 5 μm)色谱柱。以甲醇-0.2%磷酸溶液(45:55)为流动相;流速为1.0 ml/min;检测波长为375 nm。结果 鼠曲草中的槲皮素含量测定方法在10.22~51.10 μg/ml浓度范围内与峰面积的线性关系良好(r=0.999 7);平均回收率为100.18%,RSD为1.38%。结论 所建立的方法准确、灵敏、重现性好,可用于鼠曲草的质量控制。 |
| 中文关键词:鼠曲草 槲皮素 高效液相色谱法 |
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| Determination of quercetin in Gnaphalium affine |
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| Abstract:Objective To establish the determination method of Quercetin content in Gnaphalium affine D. Don. Methods VP-ODS Cl8(4.6 mm×150 mm, 5 μm) colmnn was used, the mobile phase was methanol-0.2% phosphoric acid solution(45:55) with the flow rate as 1.0 ml/min. The detection wavelength was 375 nm. Results The linear relation of hyperin was good in the range from 10.22 μg/ml to 51.10 μg/ml. The average recovery was 100.18%, RSD was 1.38%. Conclusion The method established was accurate, sensitive and good in reproducibility which could be applied to the quality control of G. affine D. Don. |
| keywords:Gnaphalium affine quercetin HPLC |
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