| 半支莲主要化学成分的HPLC-TOF/MS分析 |
| 投稿时间：2012-09-05 修订日期：2012-09-27 点此下载全文 |
| 引用本文：纪松岗,刘悦,毛飞,李祥,柴逸峰.半支莲主要化学成分的HPLC-TOF/MS分析[J].药学实践杂志,2012,30(6):437~439,472 |
| 摘要点击次数: 2937 |
| 全文下载次数: 1997 |
|
|
| 中文摘要:目的 通过高效液相-飞行时间质谱(HPLC-TOF/MS)联用技术,对中药半枝莲的主要化学成分进行有效的鉴定。方法 色谱分离采用SHISEIDO CAPCELL PAK C18柱(100 mm×3.0 mm, 3 μm),流动相分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.6 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果 在优化的液质联用条件下,通过飞行时间质谱鉴定出半枝莲中15个成分。结论 通过HPLC-TOF/MS联用技术,为鉴定半枝莲中的化学成分建立起了一种快速、高效的分析方法。 |
| 中文关键词:半枝莲 高效液相-飞行时间质谱 化学成分 |
| |
| Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS |
|
|
| Abstract:Objective To analyze chemical constituents of Scutellaria barbata D.Don by high pressure liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A SHISEIDO CAPCELL PAK C18 (100 mm×3.0 mm, 3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode.Results Under optimized condition, 15 major constituents in Scutellaria barbata D.Don were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of Scutellaria barbata D.Don. |
| keywords:Scutellaria barbata D.Don HPLC-TOF/MS chemical constituents |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
