| 灌胃α-硫辛酸对豚鼠噪声性听力损伤的防护作用研究 |
| 投稿时间：2011-11-24 修订日期：2012-01-11 点此下载全文 |
| 引用本文：王希营,朱全刚,高静,高申,潘勇华,苗鹏.灌胃α-硫辛酸对豚鼠噪声性听力损伤的防护作用研究[J].药学实践杂志,2012,30(2):103~106 |
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| 基金项目:国家十二五"重大新药创制"专项(2011ZXJ09106-07B). |
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| 中文摘要:目的 观察自由基清除剂α-硫辛酸对噪声性听力损伤的保护作用。方法 24只体重为250~300 g的雄性纯白色豚鼠,按体重随机分为α-硫辛酸低、中、高剂量组(n=3×6)、噪声对照组(n=6)。各组动物暴露倍频程噪声(4 kHz中心频率)115 dB SPL(sound pressure level,声压级) 4 h。α-硫辛酸组动物暴露前2 d、暴露当天至暴露后7 d连续灌胃给予α-硫辛酸30 mg/(kg·d)(低剂量组)、60 mg/(kg·d)(中剂量组)、120 mg/(kg·d)(高剂量组);对照组动物相应时间灌胃给予等量的生理盐水。暴露后不同时间(3、7、10 d)测试各组动物在不同频率(8、16、24、32 kHz)下的听觉脑干反应(auditory brainstem response,ABR)值,组织学检查内外毛细胞缺失和损伤程度,以测试时的听阈值与暴露前听阈差值(听阈偏移)反映听力受损情况。结果 暴露后3 d,低、中、高剂量组在8、16 kHz频率下,与噪声对照组相比较具有显著性差异;低、高剂量组在24、32 kHz频率下与噪声对照组相比较具有显著性差异;暴露后7 d,中、高剂量组在8、16、24 kHz频率下与噪声对照组相比较具有显著性差异;低、高剂量组在32 kHz频率下与噪声对照组相比较具有显著性差异;暴露后10 d,高剂量组与噪声对照组比较具有显著性差异。组织学检查,α-硫辛酸组动物无明显内外毛细胞缺失,毛细胞形态结构破坏较轻。对照组动物内耳外毛细胞显示水肿、空泡变性等病变。结论 高、中、低剂量的α-硫辛酸对噪声性听力损伤均有一定的预防作用;高剂量的α-硫辛酸对噪声性听力损伤具有很好的预防和治疗作用。 |
| 中文关键词:噪声性听力损伤 听觉脑干反应 α-硫辛酸 保护作用 预防作用 |
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| Protective effect of α-lipoic acid on noise induced hearing loss in guinea pigs |
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| Abstract:Objective To observe the protection of free radical scavenger: α-lipoic acid on the explosive hearing loss in rats. Methods 24 pure white male guinea pigs weighted between 250~300 g, which were randomly divided into α-lipoic acid low, medium, high group(n=3×6), Noise controlled group(n=6). Each group of animals was exposed to octave band noise (4 kHz center frequency) 115 dB SPL 4 h. α-Lipoic acid group were administered α-lipoic acid 30 mg/(kg·d), 60 mg/(kg·d), 120 mg/(kg·d) for 10 days (2 days before the exposure, 7 days after); Noise controlled group animals were given normal saline orally. After exposure, auditory brainstem response (ABRs) was tested in animals in each group at different times; hair cell loss and damage were examined through histological means. Results After exposure of 3 days, lower/middle/ high dose group had significant differences with noise control group at frequencies 8, 16 kHz; low and high dose group had significant differences with noise control group at frequencies 24, 32 kHz; 7 days after exposure, middle/ high dose group had significant differences with noise control group at frequencies of 8, 16, 24 kHz), low and high dose group had significant differences with noise control group at frequencies 32 kHz; after 10 days, high dose group had significant differences with noise control group at each frequencies. In the histological examination, animals of three α-lipoic acid dose group were found little inside and outside hair cell loss or damage. Noise controlled animals showed outer hair cells in the ear edema/vacuolar degeneration and other diseases. Conclusions Low, middle, high doses of a-lipoic acid on the explosive hearing loss had a preventive effect. High dose α-lipoic acid could take a good prevention and treatment in noise-induced hearing loss. |
| keywords:noise induced hearing loss auditory brainstem response α-lipoic acid protective effect preventive effect |
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