| 荷叶化学成分的HPLC-TOF/MS分析 |
| 投稿时间：2011-06-09 修订日期：2011-09-01 点此下载全文 |
| 引用本文：周永刚,刘畅,毛飞,李祥.荷叶化学成分的HPLC-TOF/MS分析[J].药学实践杂志,2011,29(5):342~346 |
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| 中文摘要:目的 通过高效液相-飞行时间质谱(HPLC-TOF/MS)联用技术定性分析荷叶中的主要化学成分。 方法 色谱分离采用Agilent Zorbax SB-Aq(250 mm×4.6 mm,5 μm),流动相组成分别为乙腈和1.0%醋酸,梯度洗脱,流速为1.0 ml/min,质谱定性采用飞行时间质谱,正离子模式扫描。 结果 在优化的液质联用条件下,结合数据库匹配技术和碎片离子分析,鉴定出荷叶中主要的生物碱类和黄酮类成分。 结论 建立了一种简单、可靠的HPLC-TOF/MS方法对荷叶中主要化学成分进行了鉴定。 |
| 中文关键词:荷叶 生物碱 黄酮 高效液相-飞行时间质谱 |
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| Analysis of chemical constituents of Lotus leaf by HPLC-TOF/MS |
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| Abstract:Objective To analyze chemical constituents of Lotus leaf by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A Agilent Zorbax SB-Aq column(250 mm×4.6mm,5μm) was used to separate. The mobile phase consisted of water containing 1.0 ％ ethylic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, the major constituents of alkaloids and flavones in Lotus leaf were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclution The method of HPLC-TOF/MS was established to identify the chemical constituents of Lotus leaf which was simple and reliable. |
| keywords:Lotus leaf alkaloids flavones HPLC-TOF/MS |
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