| HPLC法测定防风中升麻苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量 |
| 投稿时间：2010-07-08 修订日期：2010-09-09 点此下载全文 |
| 引用本文：李悦悦,王慧,陈俊,赵亮,张海,张国庆.HPLC法测定防风中升麻苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量[J].药学实践杂志,2010,28(6):445~447,474 |
| 摘要点击次数: 1304 |
| 全文下载次数: 1165 |
|
|
| 中文摘要:目的:建立高效液相色谱法测定防风中升麻苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量。方法:采用Agilent Zorbax SB-C18(5μm,150 mm×4.6 mm id)柱,甲醇-水流动相体系梯度洗脱,检测波长254 nm,流速0.7 ml/min,柱温25℃,进样量5μl。结果:成功建立了防风中4种主要成分的HPLC含量测定方法:,且方法学考察结果良好。结论:该方法简便可行,定量准确,可用于防风中4种主要成分的质量控制。 |
| 中文关键词:防风 升麻苷 升麻素 5-O-甲基维斯阿米醇苷 亥茅酚苷 HPLC |
| |
| Determination of prim-O-glucosylcimifugin,cimifugin,4′-O-glucosyl-5-O-methylvisamminol and sec-O-β-D-glucosylhamma-udol in Radix Saposhnikoviae by HPLC |
|
|
| Abstract:Objective To establish a HPLC method for the determination of prim-O-glucosylcimifugin,cimifugin,4'-O-glucosyl-5-O-methylvisamminol and sec-O-β-D-glucosylhammaudol in Radix Saposhnikoviae.Methods The sample was analyzed on an Agilent Zorbax SB-C18(5 μm,150 mm×4.6 mm id) column with methanol-water mobile phase for the gradient elution.The detection wavelength was 254 nm and flow rate was 0.7 ml/min.The column temperature was 25 ℃ and injection volume was 5 μl.Results The HPLC method for the determination of four major components in Radix Saposhnikoviae was established.The result of method was good.Conclusion The method was simple and feasible,and accurate which could be available for the quality control of Radix Saposhnikoviae. |
| keywords:Radix Saposhnikoviae prim-O-glucosylcimifugin cimifugin 4'-O-glucosyl-5-O-methylvisamminol sec-O-β-D-glucosylhammaudol HPLC |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
