HPLC测定宣肺清热合剂中盐酸麻黄碱与盐酸伪麻黄碱含量

刘惠军; 陈睿; 王娟

HPLC测定宣肺清热合剂中盐酸麻黄碱与盐酸伪麻黄碱含量

投稿时间：2009-12-28 修订日期：2010-03-09 点此下载全文

引用本文：刘惠军,陈睿,王娟.HPLC测定宣肺清热合剂中盐酸麻黄碱与盐酸伪麻黄碱含量[J].药学实践杂志,2010,28(3):199~200,205

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作者	单位
刘惠军	上海市松江食品药品检验所,上海201600
陈睿	上海市松江食品药品检验所,上海201600
王娟	贵阳中医学院,贵州贵阳550001

中文摘要:目的:建立宣肺清热合剂（麻黄、苦杏仁、浙贝母、黄芩等）中有效成分盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。方法:采用高效液相色谱法,色谱柱Agilent ZORBA×SB-C₁₈（5μm,4.6 mmx 250 mm）,流动相为乙腈-0.02 mol/L磷酸二氢钾（用磷酸调pH至2.7）,（4:100,含0.1%三乙胺）,流速:1.2 ml/min,检测波长:207 nm。结果:盐酸麻黄碱在11.04-222.8μg/mL（r=0.999 9）,盐酸伪麻黄碱在8.27-165.4μg/ml（r=0.999 9）的浓度范围内均线性关系良好,回收率均在95%-105%之间,RSD均小于3%。结论:本方法操作简便、重现性好,可以更有效地控制制剂的质量。

中文关键词:宣肺清热合剂 HPLC 盐酸麻黄碱盐酸伪麻黄碱

Determination of ephedrine and pseudoephedrine in Xuanfei Qingre mixture by HPLC

Abstract:Objective To set up a method of simultaneously determining the contents of ephedrine and pseudoephedrine in Xuanfei Qingre mixture(Herba Ephedrae,Semen Armeniacae amarum,Bulbus Fritillaria thunbergii,Radix scutellaria,etc).Methods The HPLC-quantitative analysis was carried out on a column of Agilent ZORBA× SB-CC₁₈ by using a mobile phase of acetonitrile-0.02 mol/L potassium dihydrogen phosphate(adjusted with phosphoric acid pH=2.7),(4100,with 0.1% triethylamine)under a flow rate of 1.2 ml/min.207 nm was selected as the wavelength of detector.Results The linear range of ephedrine was 11.04～222.8 μg/ml(r=0.999 9)and pseudoephedrine's was 8.27～165.4 μg/ml(r=0.999 9).The recoveries of them were all between 95% and 105%(RSD<3%).Conclusion This method is simple,reproducible and specific.

keywords:Xuanfei Qingre mixture HPLC ephedrine hydrochloride pseudoephedrine hydrochloride

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