| 液质联用方法测定白色念珠菌中海藻糖的含量 |
| 投稿时间：2010-01-25 修订日期：2010-03-08 点此下载全文 |
| 引用本文：董玲玲,曹颖瑛,柴逸峰.液质联用方法测定白色念珠菌中海藻糖的含量[J].药学实践杂志,2010,28(3):186~188 |
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| 中文摘要:目的:采用液质联用方法测定白色念珠菌中海藻糖的含量,考察白色念珠菌在感应分子法尼醇作用后海藻糖的变化。方法:色谱柱:Shisheido CAPCELL NH2（150 mm×4.6 mm,5μm）,柱温:25℃,流动相:乙腈-水溶液（75:25,V/V）,流速:1ml/min,分流比3:1。质谱检测器,ESI（-）模式,选择离子检测,碎片电压120 V。结果:海藻糖和内标峰形均良好,海藻糖含量在0.05-50μg/ml范围内线性关系良好,r=0.999 3。日内日间精密度RSD值分别为1.8%和2.1%。结论:该方法简便易行,选择内标之后含量测定更精确,灵敏度提高。 |
| 中文关键词:海藻糖 液质联用 白色念珠菌 含量测定 |
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| Determination of trehalose in Candida albicans by HPLC-MS |
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| Abstract:Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans.Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used.Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %.Conclusion This method is a simple method for quantification of trehalose in fungus. |
| keywords:trehalose LC-ESI-MS Candida albicans quantification |
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