| HPLC-ELSD法测定瓜子金中瓜子金皂苷己的含量 |
| 投稿时间：2009-10-22 修订日期：2009-12-14 点此下载全文 |
| 引用本文：焦晴晴,汪维云,苏娟,张卫东.HPLC-ELSD法测定瓜子金中瓜子金皂苷己的含量[J].药学实践杂志,2010,28(2):137~139 |
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| 中文摘要:目的:建立测定瓜子金皂苷己的含量的法,为瓜子金药材质量控制提供依据。方法:采用HPLC-ELSD法;色谱柱:SHISEIDO Capcell Pak C18柱;流动相:乙腈-水（30:70）,等度洗脱;流速:1.0 ml/min;柱温:25℃,检测器:蒸发光散射检测器。结果:瓜子金中瓜子金皂苷己获得良好分离,在0.346-3.460μg范围内呈现良好线性关系;精密度试验RSD为1.64%;重现性试验RSD为2.12%;加样回收率为99.85%。结论:建立了HPLC-ELSD法测定瓜子金皂苷己的含量,方法简单可行。 |
| 中文关键词:瓜子金皂苷己 高效液相色谱 蒸发光散射检测器 |
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| Determination of polygalasaponin F in Polygala japonica by HPLC-ELSD method |
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| Abstract:Objective To establish an effective quality control method of the traditional Chinese medicine——Polygala japonica Houtt.Methods HPLC-ELSD method was adopted for the determination of polygalasaponin F in Polygala japonica Houtt.The analysis was performed on a SHISEIDO Capcell Pak C18(4.6 mm×250 mm,5 μm) as the analytical column,acetonitrile-water(3070,v/v) as the mobile phase.The flow rate was 1.0 ml/min.The column temperature was at 25 ℃.Results The validation was carried out and the linearity(r2 = 0.999 8) between 0.346 μg and 3.460 μg and recovery(99.85%) were acceptable.The precision and reproducibility were evaluated with RSD values 1.64% and 2.12%,respectively.Conclusion This method is simple and feasible for the determination of polygalasaponin F in Polygala japonica Houtt. |
| keywords:Polygalasaponin F HPLC ELSD |
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