| HPLC法测定土鳖虫中尿嘧啶和尿囊素的含量 |
| 投稿时间：2009-09-06 修订日期：2009-11-30 点此下载全文 |
| 引用本文：景晶,曹红.HPLC法测定土鳖虫中尿嘧啶和尿囊素的含量[J].药学实践杂志,2010,28(2):134~136 |
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| 中文摘要:目的:建立土鳖虫药材中尿嘧啶和尿囊素的含量测定方法。方法:采用高效液相色谱法,色谱柱为AlltimaNH2（150 mm×4.6 mm,5μm）;流动相为乙腈-水（90:10）;流速为1.0 ml/min,检测波长205 nm;柱温35℃。结果:尿囊素和尿嘧啶的线性范围分别是:0.404-3.23μg（r=0.999 5,n=5）,0.820-6>.56μg（r=0.999 5,n=5）。平均回收率分别为98.8%、99.0%,RSD分别为0.96%、0.79%（n=6）。结论:该方法简便、准确,可作为土鳖虫药材的质量控制方法。 |
| 中文关键词:土鳖虫 尿嘧啶 尿囊素 高效液相色谱 |
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| Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC |
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| Abstract:Objective To establish a method for determination of uracil and auantion in Eupolyphaga seu Steleophaga by HPLC.Methods The HPLC separation was performed on Alltima NH2(150 mm×4.6 mm,5 μm)column with a mixture of acetonitrile-water(9010)as the mobile phase,at a flow rate of 1.0 ml/min.Detection wavelength was at 205 nm,and column temperature was at 35 ℃.Results The linear response of Auantion and Uracil range from 0.404～3.23 μg(r=0.999 5,n=5)and 0.820～6.56 μg(r=0.999 5,n=5);the average recovery was 98.8% with RSD 0.96% and 99.0% with RSD 0.79% respectively(n=6).Conclusion The method is simple,accurate and suitable for the determination of auantion and uracil in Eupolyphaga seu Steleophaga. |
| keywords:Eupolyphaga seu Steleophaga auantion uracil HPLC |
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