| 中药奇蒿提取物体外抗菌活性的实验研究 |
| 投稿时间：2009-05-04 修订日期：2009-12-24 点此下载全文 |
| 引用本文：谭蔚锋,王靖,邢新,秦路平.中药奇蒿提取物体外抗菌活性的实验研究[J].药学实践杂志,2010,28(2):101~104 |
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| 基金项目:军队“十五”青年基金资助项目(01Q085) |
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| 中文摘要:目的:研究中药奇蒿80%乙醇粗提取物及其石油醚、氯仿、乙酸乙酯、正丁醇提取物对临床常见菌种的抗菌活性。方法:对中药奇蒿进行系统性分离提取,以80%乙醇粗提取物和石油醚、氯仿、乙酸乙酯、正丁醇提取物进行体外药敏实验,序贯使用纸片扩散法和常量肉汤稀释法,选用临床常见的致病菌株,包括金黄色葡萄球菌(标准型ATCC25923)、大肠埃希氏菌(标准型ATCC25922)、铜绿假单胞菌(标准型ATCC27853)、无乳链球菌(临床型)、粪肠球菌(临床型)、福氏志贺菌(临床型)和痢疾志贺菌(临床型)。结果:奇蒿80%乙醇粗提取物对痢疾志贺菌的最小杀菌浓度(MBC)为25 mg/ml;奇蒿氯仿提取物对大肠埃希氏菌的MBC为6.25 mg/ml,对金黄色葡萄球菌的MBC为12.5 mg/ml;奇蒿乙酸乙酯提取物对福氏志贺菌、痢疾志贺菌、无乳链球菌的MBC均为6.25 mg/ml,对金黄色葡萄球菌的MBC为12.5 mg/ml;奇蒿正丁醇提取物对无乳链球菌的MBC为12.5 mg/ml,对痢疾志贺菌的MBC为25 mg/ml;石油醚提取物未对测试的细菌表现出明显的抗菌活性。结论:奇蒿不同提取物对临床多种致病菌表现出了良好的杀菌作用,在抗感染治疗领域具有较好的开发和应用前景,其有效单体及杀菌机制有待进一步研究发现。 |
| 中文关键词:奇蒿 提取物 体外抗菌实验 最小杀菌浓度 |
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| Antibiotic tests of different polar extracts of Artemisia anomala in vitro |
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| Abstract:Objective The antibiotic tests of different polar extracts of Artemisia anomala S.Moore in vitro.Methods The antibiotic tests of different polar extracts of AASM in vitro were performed by using Kirby-Bauer method and broth dilution method.Results The minimal bactericidal concentration(MBC) of 80% ethyl alcohol extract of Artemisia anomala S.Moore is 25 mg/ml for Shigella dysenteriae.The MBC of chloroform extract is 6.25 mg/ml for Escherichia coli.The MBC of chloroform extract is 12.5 mg/ml for staphylococcus aureus.The MBC of acetic ether extract is 6.25 mg/ml for Shigella flexneri,Shigella dysenteriae and Streptococcus agalactiae.The MBC of acetic ether extract is 12.5 mg/ml for staphylococcus aureus.The MBC of butanol extract of is 12.5 mg/ml for Streptococcus agalactiae.The MBC of butanol extract is 25 mg/ml for Shigella dysenteriae.Petroleum ether extract did not show significant antibacterial activity.Conclusion Different polar parts of Artemisia anomala S.Moore on a variety of clinical pathogenic bacteria showed good bactericidal effect in vitro.Thus,at the anti-infective therapeutic areas there are great prospects for development and application.The effective monomer of Artemisia anomala and its bactericidal mechanism needs further study. |
| keywords:Artemisia anomala extracts antibiotic test minimal bacterial concentration(MBC) |
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