反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量

王曙东; 潘璟; 方李; 夏曙辉; 宋炳生

反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量

投稿时间：2009-04-22 修订日期：2009-10-16 点此下载全文

引用本文：王曙东,潘璟,方李,夏曙辉,宋炳生.反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量[J].药学实践杂志,2010,28(1):45~47

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作者	单位
王曙东	南京军区南京总医院制剂科,江苏南京210002
潘璟	南京军区南京总医院制剂科,江苏南京210002
方李	南京军区南京总医院制剂科,江苏南京210002
夏曙辉	南京军区南京总医院制剂科,江苏南京210002
宋炳生	南京军区南京总医院制剂科,江苏南京210002

基金项目:南京市科技发展计划资助项目(20028006)

中文摘要:目的建立反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因含量的测定方法。方法采用Hedera-ODS-3 C₁₈色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%磷酸溶液(12:88)为流动相,三乙胺调节pH3.0±0.1,检测波长为254 nm。结果诺氟沙星和盐酸利多卡因在0.025~0.100 mg/ml浓度范围内均有良好线性关系。诺氟沙星平均回收率为98.84%,RSD为1.72%,盐酸利多卡因平均回收率为100.24%,RSD为1.86%。结论该法简便准确,稳定无干扰,重现性好,可用作壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量测定。

中文关键词:壳糖止血海绵诺氟沙星盐酸利多卡因高效液相色谱

Determination of lidocaine hydrochloride and norfloxacine in chitosan hemostatic sponge by RP-HPLC

Abstract:Objective To establish a quantitative determination method for lidocaine hydrochloride and norfloxacine in chitosan hemostatic sponge by RP-HPLC.Methods The determination was carried out with a Hedera-ODS-3 C₁₈ column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile-1%phosphoric acid solution(12:88),with pH adjusted to 3.0±0.1 by using triethylamine.Detecting wavelength was at 254 nm.Results The linear range for lidocaine hydrochloride and norfloxacine was both 0.025～0.100 mg/ml.The average recovery and RSD of norfloxacine were 98.84% and 1.72%,and those of lidocaine hydrochloride were 100.24% and 1.86%.Conclusion The method offers simple instrumental operation,good stability,and high accuracy for the determination of the chitosan hemostatic sponge.

keywords:chitosan hemostatic sponge lidocaine hydrochloride norfloxacine HPLC

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