| 反相高效液相色谱法测定芩蓝滴鼻液中黄芩苷含量 |
| 投稿时间：2009-04-22 修订日期：2009-10-19 点此下载全文 |
| 引用本文：陈维中.反相高效液相色谱法测定芩蓝滴鼻液中黄芩苷含量[J].药学实践杂志,2010,28(1):42~44 |
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中文摘要:目的建立反相高效液相色谱法分离测定芩蓝滴鼻液中黄芩苷的含量。方法:采用Gemini C18110A(4.60 mm×250 mm,5μm)色谱柱;流动相为甲醇-0.2%磷酸溶液(50:50);流速为0.8 ml/min;检测波长为277 nm;进样量10μl。结果黄芩苷浓度在709.4~7094.0μg/ml范围内与峰面积呈良好的线性关系,r=0.999 9;平均回收率为99.6%,RSD为1.33%(n=9)。结论本方法简便、准确、专属性强,可用于测定芩蓝滴鼻液中黄芩苷的含量。 |
| 中文关键词:反相高效液相色谱法 芩蓝滴鼻液 黄芩苷 含量 |
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| Quantitative analysis of baicalin in Qinlan nasal drops by RP-HPLC |
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| Abstract:Objective To develop RP-HPLC method for the determination of baicalin in Qinlan nasal drops.Methods The chromatographic analysis was performed on Gemini C18 110A(4.60 mm×250 mm,5 μm)column.The mobile phase was consisted of methanol-0.2% phosphoric acid solution(50: 50).The flow rate was 0.8 ml/min.The detection wavelength was set at 277 nm,with injection volume 10 μl.Results A good linearity was obtained over the range of 709.4～7094.0μg/ml(r=0.999 9).The average recovery of baicalin was 99.6%,with RSD 1.33%(n=9).Conclusion The method is simple,accurate,specific and will be used for the quality control of Qinlan nasal drops. |
| keywords:RP-HPLC Qinlan nasal drops baicalin content |
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