| 重楼总皂苷的纯化工艺研究 |
| 投稿时间：2009-07-08 点此下载全文 |
| 引用本文：孙治国,张琳,田景奎,钟延强,鲁莹.重楼总皂苷的纯化工艺研究[J].药学实践杂志,2009,27(6):455~458,469 |
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| 中文摘要:目的:优选重楼总皂苷的纯化工艺。方法:综合运用醇溶液调节pH、醇提和大孔吸附树脂技术,以总皂苷含量、总皂苷收率为指标,优选重楼总皂苷的纯化工艺。结果:优化后的重楼总皂苷的纯化工艺条件为:70%乙醇提取液调节pH值至9,抽滤,将滤液调pH值至中性,浓缩至0.5 g生药/mL,调pH值至8,离心(4000 r/min,30 min),上清液部分上D101大孔树脂柱纯化(上样量为70%饱和吸附量,径高比为1:5,上样流速为4.5 BV/h,纯化溶剂为20%乙醇,用量为6 BV,洗脱溶剂为70%乙醇,用量为8 BV,流速为7 BV/h)。采用本工艺制备的中间体中总皂苷含量达71.6%,总皂苷收率为85.71%。结论:验证试验结果表明,优选出的工艺稳定、合理、可行。 |
| 中文关键词:重楼 总皂苷 纯化工艺 |
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| Studies on purification process of steroidal saponins from Paris polyphylla |
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| Abstract:Objective:To optimize the purification process of saponins from Paris polyphylla Smith.Methods:According to the purity and gaining rate of total saponins,the optimum purifying process was obtained by a combination of adjusted the pH in alcohol solution,alcohol extracting-water precipitating and macroporous resin techniques.Results:The optimum purification conditions were obtained as follows:70% alcohol solution,the pH of which was adjusted to 9,was suckingly filtrated.After the pH to 7,the filtrate was concentrated to 0.5 g/mL and then centrifuged(4 000 r/min,30 min).The clear supernatant liquid was purified by D101 macroporous resin(loading sample volume:70% adsorption capacity,column diameter vs height:15,loading flow rate:4.5 BV/h,purification solvent:20% alcohol,volume:6 BV,eluting solvent:70% alcohol,volume:8 BV,eluting flow rate:8 BV/h).The purity and gaining rate of total saponins prepared by the optimum process were 71.6% and 85.71% respectively.Conclusion:The purification process of saponins obtained by this experiment is steady,reasonable and feasible. |
| keywords:Paris polyphylla total saponins purification process |
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