| 3种常用抗癌药物对人肝癌细胞HepG2抑制作用的研究 |
| 投稿时间：2009-02-25 点此下载全文 |
| 引用本文：贾静,李悦悦,朱臻宇,柴逸峰,张国庆.3种常用抗癌药物对人肝癌细胞HepG2抑制作用的研究[J].药学实践杂志,2009,27(5):323~333,361 |
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| 中文摘要:目的:研究3种常用抗癌药物对于HepG2细胞的抑制情况。方法:细胞铺96孔板,3种抗癌药物以不同浓度给药,每浓度设3复孔,不加药组做空白对照,MTT法测定,以抑制率对药物浓度作图。结果:在1～420μg/mL浓度范围内,5-氟尿嘧啶抑制效果最差;20～300μg/mL丝裂霉素C抑制效果好于长春新碱;1～20μg/mL和300～420μg/mL浓度范围内,长春新碱抑制效果好于丝裂霉素C。结论:该方法评价对比了3种常用的抗癌药物对于人肝癌细胞的抑制效果,为药物筛选中阳性药物的选择提供一定借鉴。 |
| 中文关键词:MTT HepG2 半数抑制浓度 5-氟尿嘧啶 硫酸长春新碱 丝裂霉素C |
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| Inhibition research of three commonly-used anti-tumor drugs against HepG2 cell line |
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| Abstract:Objective:To establish an inhibition research method of three commonly-used anti-tumor drugs against HepG2 cell line.Methods: The determination was performed on a 96-well plate.The drugs were administrated in a series of concentrations,and each concentration was triplicate.Cells without drugs were set as a negative control.Followed by determination of MTT method,the inhibition ratio was plotted to concentration.Results:The inhibition ability of 3 drugs were different when the concentration were in different range.On the whole,the inhibition ability of 5-Fu was the weakest one.Conclusion:The method evaluates and compares the inhibition ability of 3 commonly-used anti-tumor drugs against HepG2 cell line,and could be a reference in positive control of drug screening experiment. |
| keywords:MTT HepG2 IC50 5-Fu Vincristine Mitomycin C |
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