| 柱前衍生化HPLC测定人纤维蛋白粘合剂中盐酸精氨酸和亮氨酸的含量 |
| 投稿时间：2008-12-31 点此下载全文 |
| 引用本文：赵艳红,王强,徐立.柱前衍生化HPLC测定人纤维蛋白粘合剂中盐酸精氨酸和亮氨酸的含量[J].药学实践杂志,2009,27(3):198~200 |
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| 中文摘要:目的:建立HPLC法测定人纤维蛋白粘合剂中盐酸精氨酸和亮氨酸的含量。方法:采用Shim-pack CLC-ODS色谱柱,柱前衍生化,流动相A为50%乙腈,流动相B为醋酸盐缓冲液;检测波长362 nm;用外标法检测。结果:本色谱条件下2种氨基酸衍生物能良好地分离,衍生化试剂对氨基酸分析无干扰。平均回收率:盐酸精氨酸为102.8%,RSD为0.83%;亮氨酸为103.8%,RSD为0.92%。结论:本方法准确,方便,费用低,可用于人纤维蛋白粘合剂中2种氨基酸含量的控制。 |
| 中文关键词:盐酸精氨酸 亮氨酸 高效液相色谱法 衍生化 2,4-二硝基氟苯 |
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| Determination of two amino acids in human fibrin sealant kit by pre-column derivatization HPLC |
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| Abstract:Objective:To set up a method for determination of arginine and leucine in human fibrin sealant kit.Methods:A Shim-pack CLC-ODS column was used after pre-column derivatization with 2,4-dinitrobenzene fluoride,with the mobile phase of 50% acetonitrile and 0.05mol/L acetate buffer,and the UV detection wavelength was 362 nm.The content was analyzed by external standard method.Results:These two amino acid derivatives can be well separated under the chromatographic conditions.The average recovery was 102.8%(RSD=0.83%) for Arg,and 103.8%(RSD=0.92%) for Leu.Conclusion:The method is accurate,convenient,low-cost and suitable for determination of the two amino-acids in human fibrin sealant kit. |
| keywords:arginine hydrochloride leucine HPLC derivation 2,4-dinitrofluorobenzene |
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