| 砷剂对人胃癌细胞凋亡及LRP耐药基因表达影响的研究 |
| 投稿时间：2008-10-06 点此下载全文 |
| 引用本文：耿礼文,薄挽澜,梁桃.砷剂对人胃癌细胞凋亡及LRP耐药基因表达影响的研究[J].药学实践杂志,2009,27(2):129~131 |
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| 中文摘要:目的:研究三氧化二砷(As2O3)对人胃癌细胞的诱导凋亡作用及对LRP、C-myc基因表达的影响。方法:选用人胃癌BGC-823细胞株,运用体外细胞培养法,MTT法,流式细胞术检测As2O3对人胃癌的诱导凋亡作用;用逆转录-聚合酶链反应(RT-PCR)方法检测LRP、C-myc mRNA的表达。结果:As2O3对人胃癌BGC-823细胞具有抑制作用,其抑制率呈时间-剂量依赖关系。不同浓度的As2O3均可诱导凋亡。1.0、2.0μmol/L的As2O3可下调LRP、C-myc的表达。结论:As2O3具有抗肿瘤作用,主要是通过诱导细胞凋亡实现的,其机制与下调LRP、C-myc表达有密切关系。 |
| 中文关键词:三氧化二砷 胃癌细胞 LRP C-myc 凋亡 |
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| Effect and mechanism of arsenic trioxide on LRP expression on human gastric carcinoma cells |
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| Abstract:Objective:To study the apoptosis and mechanism of arsenicals on human gastric carcinoma cells.Methods: MTT,FCM and RT-PCR tests were used to investigate of apoptosis levels induced by arsenic trioxide and the effect on LRP、C-myc expression.Results:1.0 μmol/L and 2.0 μmol/L arsenic trioxide can decrease LRP、C-myc expression.Conclusions:Low concentrations may improve apoptosis of gastric cancer by decreasing LRP、C-myc expression.As2O3 may be used to treat gastric cancer but the results are no better than cisplatin. |
| keywords:arsenic trioxide gastric cancer apoptosis LRP,C-myc |
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