| 聚酰胺柱层析法分离回心草总黄酮的研究 |
| 投稿时间：2008-06-18 点此下载全文 |
| 引用本文：蔡鹰,陆晓和,魏群利.聚酰胺柱层析法分离回心草总黄酮的研究[J].药学实践杂志,2009,27(1):58~60 |
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| 中文摘要:目的:确定聚酰胺柱层析法分离回心草总黄酮的条件。方法:对聚酰胺用量、乙醇洗脱浓度、洗脱速度等方面进行考察。结果:干法上样,聚酰胺用量以药材:聚酰胺=1:1为佳,以50%的乙醇为洗脱溶剂,2倍柱体积/h为最佳的洗脱速率。结论:聚酰胺可较好地用于回心草总黄酮的分离富集。 |
| 中文关键词:回心草 聚酰胺 总黄酮 |
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| Studies of isolation for total flavonoids in Rhodobryum giganteum by polyamide column adsorption chromatogtaphy |
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| Abstract:Objective: To select the optimal condition of polyamide column adsorption chromatogtaphy for separating the total flavonoids in Rhodobryum giganteum(Schwaegr.) Par.Methods:The amount of polyamide,the eluting reagent and the eluting rate were studied.Results:It was suitable when the herb amount:polyamide amount was 11,and 50% ethnoal was the sutiable eluting reagent.The flow rate of eluent was 2 times volume of column/h.Conclusion:polyamide is suitable for the separation and collection of the total flavonoids from Rhodobryum giganteum(Schwaegr.) Par. |
| keywords:Rhodobryum giganteum(Schwaegr.) Par. polyamide total flavonoids |
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