| 毛细管电泳法检查左旋麻黄碱中对映异构体杂质的研究 |
| 投稿时间：2008-10-14 点此下载全文 |
| 引用本文：刘长海,赵亮,张海,朱臻宇,张国庆,柴逸峰.毛细管电泳法检查左旋麻黄碱中对映异构体杂质的研究[J].药学实践杂志,2009,27(1):33~37 |
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| 基金项目:国家“十一五”科技支撑计划课题(2006BAI08B03-07) |
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| 中文摘要:目的:建立左旋麻黄碱中对映异构体杂质检查的毛细管电泳方法。方法:以SBE-β-CD和HP-β-CD二元环糊精为手性添加剂,探讨了环糊精种类及浓度、pH、有机溶剂种类等因素对麻黄碱及伪麻黄碱对映体拆分的影响,结果表明:毛细管:未涂渍熔融石英毛细管75μm×68 cm(有效长度60 cm)。分离用缓冲液为25 mmol/L的Tris缓冲液,含2.0%HP-β-CD和1.0%SBE-β-CD,用磷酸调节pH至3.0。分离电压16kV;温度15℃;虹吸进样:高度10 cm,时间3 s;紫外检测波长214 nm。结果:麻黄碱及伪麻黄碱对映体得到良好分离,并满足进行左旋麻黄碱中对映异构体杂质检查所需要的条件。结论:该方法简单可靠、专属性强,可以作为常规的杂质检查手段。 |
| 中文关键词:麻黄碱 对映体分离 毛细管电泳法 环糊精 杂质检查 |
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| Analysis of optical impurity of ephedrine levorotatory by capillary electrophoresis |
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| Abstract:Objective: To develop a method for purity test and enantioseparation of ephedrine levorotatory by capillary electrophoresis(CE).Methods: The cyclodextrin,concentration,pH,organic solvent were studied,and the results indicated that the enantiomer of ephedrin and ephedrine levorotatory were baseline separated under the following experimental conditions:uncoated fused silica capillary(75 μm × 68 cm),25 mmol/L Tris,2% HP-β-CD and 1% SBE-β-CD,pH was adjusted at 3.0 by phosphoric acid,and 15 kV separation voltage.The detection wavelength was set at 214 nm with the column temperature of 15 ℃.Results: Under the experimental conditions adopted,the baseline separation of ephedrin and ephedrine levorotatory was obtained.Conclusions: The proposed method is simple,reliable and can be used for routine purity test and chiral separation. |
| keywords:ephedrin enantioseparation capillary electrophoresis(CE) cyclodextrin purity test |
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