| HPLC法测定林可霉素乳膏中盐酸林可霉素的含量 |
| 投稿时间：2007-10-15 点此下载全文 |
| 引用本文：朴淑娟,张纯,江春霞,林厚文,陈万生.HPLC法测定林可霉素乳膏中盐酸林可霉素的含量[J].药学实践杂志,2008,(5):357~358,361 |
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| 中文摘要:目的:建立高效液相色谱法测定林可霉素乳膏中盐酸林可霉素的含量。方法:以YMC C18（250 mm×4.6 mm,5μm）为色谱柱,0.05 mol/L硼砂溶液（磷酸溶液调节pH值至6.0）-甲醇（55:45）为流动相,紫外检测波长为214 nm。结果:盐酸林可霉素在10～30μg范围内线性关系良好（r=0.999 7）,平均回收率为98.74%,（RSD=2.06%,n=9）。3批林可霉素乳膏中盐酸林可霉素的标示含量分别为96.21%、96.50%、93.38%。结论:本方法操作简便、快速,结果准确可靠,适用于林可霉素乳膏的质量控制。 |
| 中文关键词:盐酸林可霉素 乳膏 高效液相色谱法 |
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| Determination of lincomycin hydrochloride in lincomycin cream by HPLC |
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| Abstract:Objective:To establish high performance liquid chromatography method for determination the content of lincomycin hydrochloride in lincomycin cream.Methods:A YMC C18（250 mm×4.6 mm,5μm）column was used with the mobile phase of 0.05mol/L borax （adjust pH to 6.0 with phosphoric acid）-methanol （55:45）and UV wavelengh of 214 nm.Results:The calibration curve of lincomycin hydrochloride was line in the range of 10～30μg（r=0.999 7）.The average recovery was 98.74%（RSD= 2.06%,n=9）.The relative content of lincomycin hydrochloride in three batch of lincomycin cream was 96.21%,96.50% and 93.38%.Conclusion:This method is simple,quick,accurate and suitable for the quality control of lincomyein cream. |
| keywords:lincomycin hydrochloride cream HPLC |
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