| HPLC测定封管用枸橼酸钠注射液中枸橼酸盐的含量 |
| 投稿时间：2007-09-27 点此下载全文 |
| 引用本文：樊蓉,张纯,关皓月,朴淑娟.HPLC测定封管用枸橼酸钠注射液中枸橼酸盐的含量[J].药学实践杂志,2008,(5):354~356 |
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| 中文摘要:目的:建立封管用枸橼酸钠注射液中枸橼酸盐的含量测定方法。方法:采用HPLC法,选用Diamonsil C18色谱柱,以甲醇-0.05 mol/L磷酸二氢钾（pH调至2.5～2.8）（5:95,v:v）为流动相,流速为1.0 mL/ min,紫外检测波长210 nm。结果:枸橼酸盐在0.5～6 mg/mL范围内呈良好的线性关系（r=0.999 9,n= 5）;平均加样回收率为98.79%（RSD=1.08%）;3批封管用枸橼酸钠注射液中的枸橼酸盐标示含量分别为100.51%、98.22%、97.78%。结论:本方法操作简便、快速、结果准确、可靠,适用于该制剂中枸橼酸盐的含量测定。 |
| 中文关键词:高效液相色谱法 枸橼酸盐 含量测定 |
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| Content determination of citrate in sodium citrate injection for catheter locking by HPLC |
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| Abstract:Objective:To establish a content determination method of citrate in sodium citrate injection for catheter locking. Methods:HPLC was used to determine citrate on Diamonsil C18 column（250 mm×4.6 mm,5μm）,using MeOH:0.05 mol/L KH2PO4（adjusted to pH 2.5～2.8 by H3PO4）（5:95,v:v）as a mobile phase with a flow rate at 1.0 mL/min and UV wavelengh of 210 nm.Results:The calibration curve of citrate was line in the range of 0.5～6 mg/mL（r=0.999 9,n=5）.The average recovery was 98.79% （RSD=1.08%）.The relative contents of citrate in three batch of citrate in sodium citrate injection for catheter locking were 100.51%,98.22% and 97.78%,respectively.Conclusion:This method was simple,rapid,accurate and suitable for the quality control of citrate. |
| keywords:HPLC citrate content determination |
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