| 兴奋性氨基酸受体所介导细胞钙电流特征及其中L-SOP的作用途径 |
| 投稿时间：2008-07-08 点此下载全文 |
| 引用本文：张浚,王晖,金星.兴奋性氨基酸受体所介导细胞钙电流特征及其中L-SOP的作用途径[J].药学实践杂志,2008,(4):258~260,296 |
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| 基金项目:国家自然科学基金资助课题(39570827) |
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| 中文摘要:目的:研究兴奋性氨基酸受体各亚型所介导非洲爪蟾卵母细胞跨膜电流的特征及其中Ⅲ型受体激动剂L-SOP的作用途径。方法:用异硫氰酸胍-酚-氯仿法从成年大鼠脑中提取总RNA,以寡聚脱氧胸苷酸纤维素亲和层析法分离出mRNA并注射入非洲爪蟾卵母细胞使表达,以全细胞双电极电压钳位法灌流此卵母细胞。结果:100μM KA（kainate）（离子型）产生一主峰电流后缓慢下行,成为一稳定电流平台,最终脱敏,脱敏时间平均为37.57 min。2 txM QA（quisqualate）（代谢Ⅰ型）产生一峰值电流后转为一持续性钙振荡或直接出现钙振荡,振荡维持时间为（6.72±1.33）min,脱敏时间均随激动剂浓度的增加而缩短。0.8 mM L- SOP（L-phosphoserine）（代谢Ⅲ型）灌流产生规律性电流振荡,振荡时间为（20.17±8.47）min。此振荡电流可被代谢Ⅰ型受体拮抗剂L-AP3所拮抗。结论:离子型、代谢Ⅰ型及代谢Ⅲ型受体均产生各自特征电流模型;L-SOP不仅是Ⅲ型受体的激动剂,而且是Ⅰ型受体的弱的激动剂,即细胞振荡电流是L-SOP通过Ⅰ型受体的作用途径而产生。 |
| 中文关键词:非洲爪蟾卵母细胞 信使RNA 兴奋性氨基酸受体 离子型谷氨酸受体 代谢型谷氨酸受体 |
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| Characteristics of membrane current induced by excitory amino acid receptors and the acting way of L-SOP |
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| Abstract:Objective:To study the characters of membrane current of types of excitory amino acide and the acting way of TypeⅢmGluR（metabotropic glutamate receptor） agonist—L-SOP among them.Methods:A total receptor RNA was isolated from adult rat brain by acid guanidnium thiocyanate-phenol-chloroform（AGPC） method,mRNA was isolated from the total RNA by oligo（dT）-cellu- cose chromatography,and was then micro-injected into Xenopus oocytes to express,through a two-microeleetrode voltage clamp tech- nique.The ooeytes were refused with varieties of types of excitory amino acid.Results:100μM KA（kainate） （ionic glutamate recep- tor） induced first a peak current and then a slowly descending current,which desentized at last.The mean maintained time was 37.57 min.2μM QA（quisqualate） （TYPEⅠmGluR） induced regulary calcium oscillation with a maintained time of （6.72±1.33） min. Both of the maintained time were shortened the agonist concentration increased.0.8 mM L-SOP of TYPEⅢmGluR agonist induced a kind of disciplinary oscillation with a maintained time of （20.17±8.47） min which was also shortened with an increasing concentra- tion,while the oscillation coule be antagonized by TYPEⅠmGluR antagonist—L-AP3.Conclusion::Each of these three types of gluta- mate amino acid receptors has its speuniquecial current mode when irritated;L-SOP is not only an agonist of TYPEⅢmGluR,but also a weak agonist of TYPEⅠ,that is,its acting way of producing oscillation is through TYPEⅠreceptor. |
| keywords:Xenopus oocytes mRNA excitory amino acid ionic glutamate receprots metabotropic glutamate receptors KA QA L-SOP Carb |
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