| HPLC法测定复方软甲膏中水杨酸和盐酸丁卡因的含量 |
| 投稿时间：2007-07-05 点此下载全文 |
| 引用本文：顾伟锋,朴淑娟,张纯.HPLC法测定复方软甲膏中水杨酸和盐酸丁卡因的含量[J].药学实践杂志,2008,(3):217~219 |
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| 中文摘要:目的:建立测定复方软甲膏中水杨酸和盐酸丁卡因含量的HPLC法。方法:色谱柱为Agilent-C18（4.6 mm×150 mm,5μm）;流动相为甲醇-水-磷酸（50:50:0.04）,流速1.0 mL/min,检测波长313 nm。结果:水杨酸溶液在82.00-410.00μg/mL时与其吸收值呈良好线性关系（r=0.999 9）,平均加样回收率99.80%,RSD=1.68%;盐酸丁卡因溶液在5.00-25.00μg/mL时与其吸收值呈良好线性关系（r=0.999 9）,平均加样回收率99.75%,RSD=1.36%。结论:本方法操作简便、快捷,结果准确、可靠,适用于复方软甲膏的质量控制。 |
| 中文关键词:高效液相色谱法 水杨酸 盐酸丁卡因 |
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| Determination of salicylic acid and tetracation hydrochloride in Compound onychomycosis cream by HPLC |
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| Abstract:Objective:To establish a HPLC method for the determination of salicylic acid and tetracation hydrochloride in compound onychomycosis cream.Methods: Agilent-C18(4.6 mm×150 mm,5 μm) column was used with a mobile phase of methanol-water-phosphoric(50500.04) and a flow rate of l.0 mL/min,at the detection wavelength of 313 nm.Results:This method showed good linear relation(r= 0.999 9) in the range of 82.00～410.00 μg/mL for salicylic acid,and 5.00～25.00 μg/mL for tetracation hydrochloride.The average recoveries were 99.80% and 99.75%,with the relative standard deviation of 1.68% and 1.36%,respectively.Conclusion:The method is simple,quick,accurate and suitable for the quality control of compound onychomycosis cream. |
| keywords:HPLC salicylic acid tetracation hydrochloride |
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