| 反相高效液相色谱法测定醋酸氯己定器械保存液的含量 |
| 投稿时间：2007-03-27 修订日期：2007-06-18 点此下载全文 |
| 引用本文：黄金华,毛桂福,吴蓉,凌义.反相高效液相色谱法测定醋酸氯己定器械保存液的含量[J].药学实践杂志,2008,(1):48~50 |
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| 中文摘要:目的:建立反相高效液相色谱法测定醋酸氯己定器械保存液中醋酸氯己定的含量。方法:采用Shimpack CLC-C8为色谱柱,以乙腈-水（含1%三乙胺,并用醋酸调节pH至4.0）（42:58）为流动相,检测波长为258nm,流速1.0mL/min,柱温为30℃,进样体积为20μL。结果:醋酸氯己定在2080μg/mL浓度范围内线性关系良好（r=0.9998）。醋酸氯己定的平均回收率为99.96%;RSD=1.71%（n=6）。结论:本法用于测定器械保存液的醋酸氯己定含量,快速准确,结果满意。 |
| 中文关键词:醋酸氯己定 器械保存液 反相高效液相色谱法 含量测定 |
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| Determination of chlorhexidine acetate in appliance-preserving solution by RP-HPLC |
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| Abstract:Objective:To develop a RP-HPLC method for determination of chlorhexidine acetate in appliance preserving solution. Methods:RP-HPLC was used for the determination of chlorhexidine acetate in appliance's preserving solution on a Shim-pack CLC-C8 column, with acetonitrile-water （including 1% triethylamine,adjecting pH to 4.0 by glacial acetate）（42-58）as mobile phase.The detection wavelength was 258 nm,flow rate was 1.0 mL/min, column temperature was 30 ℃,sample injection volume was 20 μL. Results:Chlorhexidine acetate had a good linear coefficient （r=0.999 8） over the range of 20-80 μg/mL.The average recoveries of chlorhexidine acetate was 99.96%,RSD=1.71% （n=6）.Conclusion:This method was quick and accurate for determination of chlorhexidine acetate in appliance preserving solution. |
| keywords:chlorhexidine acetate appliance preserving solution RP-PLC determination |
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