| 高效液相色谱法测定伤科擦剂中乌头碱的含量 |
| 投稿时间：2006-05-08 点此下载全文 |
| 引用本文：张美玲.高效液相色谱法测定伤科擦剂中乌头碱的含量[J].药学实践杂志,2007,(6):391 |
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| 中文摘要:目的:建立测定伤科擦剂中乌头碱含量的高效液相色谱法。方法:采用高效液相色谱法测定。色谱柱:MN-Nucleosil 7C6H5不锈钢柱（25 cm×4.0 mm）,流动相:甲醇-水-氯仿-三乙胺（70:30:1:0.2）,流速:1.5 mL/min,在235 nm波长处紫外检测。结果:该方法线性范围为0.050-0.225μg,平均加样回收率为95.42%,RSD=1.626%（n=5）。结论:该法测定伤科擦剂中乌头碱的含量,简便、快捷、重现性好。可作为该制剂的质量控制方法。 |
| 中文关键词:伤科擦剂 乌头碱 高效液相色谱法 含量测定 |
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| Content determination of aconitine in Shangke liniment by HPLC |
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| Abstract:Objective:To set up an HPLC method for the content determination of aconitine in shangke liniment.Methods:Chromatography was performed on a MN-Nucleosil 7C6H5 stainless steel column（25 cm×4.0 mm）.The mobile phase was a mixture of methanol-water-chloroform-triethylamine（70-30-1-0.2）,with a flow rate of 1.5 mL/min.The detection wavelength was 235 nm.Results:The calibration curve was linear over the range of 0.050-0.225 μg,the average rate of recovery was 95.42 %,RSD=1.62 %（n=5）.Conclusion:The method proved to be simple,rapid,reliable and well reproducible in the content determination of aconitine in shangke liniment.It may be used as a method of quality control of the preparation. |
| keywords:Shangke liniment aconition HPLC content determination |
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