| 液相色谱法测定羟基喜树碱含量及药动学研究 |
| 投稿时间：2006-12-25 点此下载全文 |
| 引用本文：孔令媛,马骏.液相色谱法测定羟基喜树碱含量及药动学研究[J].药学实践杂志,2007,(6):379~381 |
| 摘要点击次数: 1417 |
| 全文下载次数: 45 |
|
|
| 中文摘要:目的:建立一个简单、快速、灵敏的柱切换高效液相色谱法测定人血清中10-羟基喜树碱（HCPT）含量。方法:生物样品首先在装有限进介质（RAM）填料的预柱中净化和富集,然后转移到C18分析柱分析待测物,用荧光法检测。结果:整个分析时间8 min。在1-1000 ng/mL浓度范围内,HCPT显良好的线性关系,日内、日间变异小于5%,最低检测限为0.1 ng/mL。结论:本法已应用于临床病人注射HCPT后的药代动力学研究,结果符合一房室模型并计算出它的药动学参数。 |
| 中文关键词:高效液相色谱 限进介质 柱切换 10-羟基喜树碱 药代动力学 |
| |
| HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study |
|
|
| Abstract:Objective:To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin（HCPT） in human serum.Methods: A precolumn containing restricted access media（RAM） was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively.Results: The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%.Conclusion: This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated. |
| keywords:HPLC restricted access media column-switching 10-hydroxycamptothecin pharmacokinetics |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
