HPLC法测定石韦中木犀草素的含量

张亚宁; 吴博; 杨吉刚; 胥秀英; 郑一敏

HPLC法测定石韦中木犀草素的含量

投稿时间：2005-07-03 点此下载全文

引用本文：张亚宁,吴博,杨吉刚,胥秀英,郑一敏.HPLC法测定石韦中木犀草素的含量[J].药学实践杂志,2006,(6):359~361

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作者	单位	E-mail
张亚宁	重庆工学院生物工程学院2003级本科,重庆400050	yxx651116@cqit.edu.cn
吴博	重庆工学院生物工程学院2003级本科,重庆400050
杨吉刚	重庆工学院生物工程学院2003级本科,重庆400050
胥秀英	重庆工学院生物工程学院天然药物实验室,重庆400050
郑一敏	重庆工学院生物工程学院天然药物实验室,重庆400050

中文摘要:目的：用高效液相色谱法测定石韦中木犀草素的含量。方法：采用Shimadzu C₁₈柱（150mm×4．6mm,5μm）,流动相为甲醇-0．2%H₃PO₄溶液（50：50）,流速1．0mL/min,检测波长254nm,柱温室温,用外标法定量,测定石韦中木犀草素的含量。结果：木犀草素的线性范围0．0325～0．391μg,r=0．9999,回收率95．85%,RSD为0．27%。结论：该方法简便,快速,线性关系良好,适合作为石韦中木犀草素含量测定的方法。

中文关键词:石韦木犀草素高效液相色谱法

Quantitative determination of luteolin in Pyrrosia lingua by HPLC

Abstract:Objective:To determine the content of luteolin in Pyrrosia lingua by HPLC.Methods:The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C₁₈ colunm（150mm×4.6mm,5μm）.The mobile phase was MeOH-0.2% H₃ PO₄（50:50）.Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results:The method was simple and had a good linear relationship.The liner range of luteolin is 0.40～2.00μg and gave a correlation （r）of 0.999 6.The recovery,was 95.85%,RSD 0.27%.Conclusion:The method was easy to determine the content of luteolin from Pyrrosia lingua.

keywords:Pyrrosia lingua luteolin HPLC

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