| RP-HPLC测定展筋丸中士的宁与马钱子碱的含量 |
| 投稿时间：2005-11-29 点此下载全文 |
| 引用本文：鱼爱和,张兰华,杨玉英.RP-HPLC测定展筋丸中士的宁与马钱子碱的含量[J].药学实践杂志,2006,(6):357~359 |
| 摘要点击次数: 1238 |
| 全文下载次数: 145 |
|
|
| 中文摘要:目的：建立自制中药制剂展筋丸中马钱子质量控制的方法。方法：采用RP-HPLC法测定马钱子中士的宁与马钱子碱的含量。色谱柱为XTerraTMRPC18柱；以10mmol/L碳酸氢铵溶液（浓氨水调pH至9．5） -乙腈（75：25）为流动相,检测波长为256nm,柱温35℃；展筋丸样品采用碱化后,以三氯甲烷冷浸过夜、超声处理提取。结果：线性范围士的宁为0．432～2．160μg、马钱子碱为0．388～1．940μg。士的宁回收率为100．03%,RSD为2．06%,马钱子回收率为99．41%,RSD为2．41%。结论：本法快速、准确、重现性好,可用于展筋丸中马钱子的质量控制。 |
| 中文关键词:展筋丸 马钱子 士的宁 马钱子碱 反相高效液相色谱法 |
| |
| Determination of strychnine and brucine in Zhanjin pills by RP-HPLC |
|
|
| Abstract:Objective:To establish a method for the quality control of Semen Strychni in Zhanjin pills.Methods:An RP-HPLC method for the quantitative determination of strychnine and brucine in Semen Strychni was built.A XTerra TMC18 column（150mm×3.9mm,5μm）was used with a 10mmol/L amonium bicarbonate solution（adjusted to pH 9.5 by ammonia solution）and acetonitrile（75 :25）as the mobile phase.The detective wavelength was 256nm and the column temperature was 35℃.The basifying testing sample was extracted supersonically by chloroform in cold immersion.Results:The linear ranges were 0.432～2.160μg for strychnine and 0.388～1.940μg for brucine.The average recovery and RSD were 100.03% and 2.06% for strychnine,99.41% and 2.41% for bru- cine,respectively.Conclusion:The method was specific,accurate and precise.It could be used for the quality control for Semen Strychni in Zhanjin pills. |
| keywords:Zhanjin pills Semen Strychni strychnine brucine RP-HPLC |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
