| 苦参素对胃癌细胞株MKN-54产生金属基质蛋白酶的抑制作用 |
| 投稿时间：2006-01-15 点此下载全文 |
| 引用本文：陆振东,张戈.苦参素对胃癌细胞株MKN-54产生金属基质蛋白酶的抑制作用[J].药学实践杂志,2006,(3):147~149 |
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| 中文摘要:目的:研究中药有效成分苦参素对胃癌细胞株MKN-45产生金属基质蛋白酶MMPs(matrix metallo- proteinases)的作用。方法:采用酶联免疫吸附试验检测MKN-45细胞产生金属机制蛋白酶MMP-2和 MMP-9。采用明胶酶谱法检测金属蛋白酶活性。结果:在5-20μmol／L的浓度范围内,苦参素能有效抑制 MKN-45细胞中MMP-2和MMP-9的表达,同时明胶酶谱结果显示,20μmoL／L的苦参素能够显著抑制 MMP-2和MMP-9的蛋白酶活性。结论:苦参素能够显著抑制胃癌细胞株MKN-45中金属基质蛋白酶的表达和活性,表明苦参素对胃癌转移具有一定的抑制作用。 |
| 中文关键词:苦参素 金属基质蛋白酶 胃癌 侵袭 |
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| Inhibitory effects of matrine on the production of matrix metallo - proteinases of human gastric carcinoma cell line MKN - 45 |
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| Abstract:Objective: To explore the mechanism of therapeutic effect of matrine on human gastric carcinoma by inhibiting the production of matrix metallo-proleinases of cell line MKN - 45 induced by it. Methods: The production of matrix metalloproteinases (MMP -2,MMP -9)of MKN -45 cells were measured by ELISA and gelatin zymogrmn. Results: In the dose of 5 -20μmol/L, matrine can significantly inhibit MMP-2 as well as MMP-9 expressions in MKN -45 cells. Especially, in the zymographic analysis of MMPs pxressions in conditional culture media, matrine 20μmol/L can greatly inhibit the MMP -2 and MMP -9 enzyme activity. Conclusion: Matrine could significantly inhibit the production and activity of MMPs in gastric carcinoma cell line MKN -45, which showed the inhibitory effect on the metastasis of gastric carcinoma cells. |
| keywords:matrine matrix melalloproteinases gastric carcinoma invasive |
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