| 一阶导数分光光度法测定水飞蓟宾脂质体的包封率 |
| 投稿时间：2005-03-17 点此下载全文 |
| 引用本文：张良,赵益华.一阶导数分光光度法测定水飞蓟宾脂质体的包封率[J].药学实践杂志,2006,(1):22~25 |
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| 中文摘要:目的:建立一种测定水飞蓟宾脂质体包封率的方法。方法:采用一阶导数分光光度法,中间波长为 275nm和317nm,测定波长分别为274、276、316、318nm。结果:在0-30.3μg／mL时δ与水飞蓟宾浓度呈良好的线性关系,r=0.999 5,样品的回收率100.8％,RSD=0.8％(n=9)。结论:以一阶导数分光光度法测定水飞蓟宾脂质体的包封率切实可行。 |
| 中文关键词:一阶导数分光光度法 水飞蓟宾脂质体 包封率 |
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| Determination of the entrapment efficiency of silybin liposomes by first derivative spectrometry |
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| Abstract:Objective:To establish a method for the determination of the entrapment efficiency of silybin liposomes.Methods:First derivative spectrometry was established.The two middle wavelengths were 275nm and 317nm.The detection wavelengths were 274,276, 316 and 318nm,respectively.Results:The calibration curve of silybin was linear in the range of 0-30.3uuuuuuuuuuuug/mL and the correlation coefficient was 0.9995.The average recovery and relative standard deviation were 100.8% and 0.8%,respectively.Conclusion:The method is practicable. |
| keywords:first derivative spectrometry silvbin liposomes entrapment rate |
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