| 水飞蓟宾对小鼠腹腔巨噬细胞释放纤维化因子的影响 |
| 投稿时间：2005-09-05 点此下载全文 |
| 引用本文：庄东平,王硕丰,章越凡,桂敏,张珉,张俊平.水飞蓟宾对小鼠腹腔巨噬细胞释放纤维化因子的影响[J].药学实践杂志,2006,(1):20~22 |
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| 基金项目:国家自然科学基金资助项目(No.0171087). |
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| 中文摘要:目的:研究水飞蓟宾对小鼠腹腔巨噬细胞释放纤维化因子的影响。方法:小鼠腹腔巨噬细胞先后用卡西霉素和脂多糖刺激培养24h诱导纤维化因子。巨噬细胞培养上清中促胶原合成活性和转化生长因子β活性分别采用3H-脯氨酸掺入法和雕肺上皮Mv-1-Lu细胞测定。结果:水飞蓟宾(6.25-50μg／mL)以浓度依赖方式抑制巨噬细胞产生胶原刺激活性和转化生长因子β1。结论:水飞蓟宾减少巨噬细胞释放促纤维化因子可能是其保肝抗硬化机制之一。 |
| 中文关键词:水飞蓟宾 巨噬细胞 转化生长因子 纤维化 |
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| Effect of silybin on the fibrogenic cytokines production by mouse peritoneal macrophages |
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| Abstract:Objective:To study the effect of silybin on fibrogenic cytokines production by mouse peritoneal macrophages.Methods:Mouse peritoneal macrophages were primed with calcimycin 10-6 mol/L for 8 h then elicited by lipopolysa(?)harides (LPS) 100 mg/L for 6 h to induce fibrogenic cytokines.Collagen stimulating activities and transforming growth factor β (TGFβ) activities in the macrophage culture supernatants were assessed by [3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell and [3H]-thymidine incorporation assay using Mv-1-Lu mink lung epithelial cell respectively.Results:Silybin(6.25-50(μg/mL) inhibited LPS-induced collagen stimulating activities and TGFμ production in a concentration dependent manner.Conclusion:Hepatoprotective and an(?)ifibrotic properties of silibin may partly arise from its actions on fibrogenic cytokines production. |
| keywords:silybin macrophages transforming growth factor fibrosis |
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