| 肝星状细胞HSC-T6体外肝纤维化模型的建立 |
| 投稿时间：2005-09-05 点此下载全文 |
| 引用本文：刘晔,齐荔红,王硕丰,章越凡,桂敏,张珉,张俊平.肝星状细胞HSC-T6体外肝纤维化模型的建立[J].药学实践杂志,2005,(6):339~342 |
| 摘要点击次数: 1305 |
| 全文下载次数: 1035 |
| 作者 | 单位 | | 刘晔 | 南京军区福州总院药剂科, 福建, 福州, 350001 | | 齐荔红 | 南京军区福州总院药剂科, 福建, 福州, 350001 | | 王硕丰 | 第二军医大学药学院药理学教研室, 上海, 200433 | | 章越凡 | 第二军医大学药学院药理学教研室, 上海, 200433 | | 桂敏 | 第二军医大学药学院药理学教研室, 上海, 200433 | | 张珉 | 第二军医大学药学院药理学教研室, 上海, 200433 | | 张俊平 | 第二军医大学药学院药理学教研室, 上海, 200433 |
|
| 基金项目:国家自然科学基金资助项目(No.30171087). |
|
| 中文摘要:目的 研究以肝星状细胞HSC-T6为靶细胞,建立体外肝纤维化模型.方法 小鼠腹腔巨噬细胞先后用卡西霉素和脂多糖刺激培养24h制备巨噬细胞条件培养基.肝星状细胞增殖和胶原合成分别采用结晶紫染色法和3H-脯氨酸掺入法测定.结果 血清和巨噬细胞条件培养基可显著促进HSC-T6细胞增殖与胶原合成.IL-1、TNF、EGF、FGF和PDGF均可促进HSC-T6细胞增殖,其中PDGF的促增殖能力最强.TGFβ1可剂量依赖地促进HSC-T6细胞胶原的合成.结论 用血清、巨噬细胞条件培养基、PDGF或TGFβ刺激HSC-T6细胞增殖和胶原合成作为体外肝纤维化模型是可行的. |
| 中文关键词:星状细胞 巨噬细胞 细胞因子 纤维化 |
| |
| Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells |
|
|
| Abstract:Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines. |
| keywords:hepatic stellate cells macrophages cytokine fibrosis |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
