| 雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响 |
| 投稿时间：2005-05-08 点此下载全文 |
| 引用本文：王洋,郑唯强.雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响[J].药学实践杂志,2005,(4):@ |
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| 中文摘要:目的 检测乳腺癌MCF-7细胞在雌激素(E2)和三苯氧胺(TAM)作用后BPI基因的表达情况.方法 用雌激素(E2)和三苯氧胺(TAM)处理培养的处于对数生长期的MCF-7细胞,应用MTT比色分析法筛选最佳作用浓度.以筛选的浓度同步分组作用于MCF-7细胞72h,同时设对照.用原位杂交法检测BP1基因在ER处于激活和抑制状态下的表达状况,用免疫组化检测bcl-2的表达,用TUNEL法检测细胞凋亡状况.分析MCF-7在ER处于不同表达状态下BP1、bcl-2的表达变化和细胞凋亡变化情况.结果 E2在10-8mol/L、TAM在10-6mol/L的浓度时,刺激和抑制MCF-7细胞生长的作用最显著.在E2和TAM处理后,MCF-7细胞BP1的表达率分别为95.0%±3.94%、58.5%±5.27%,bcl-2的表达率为80.9%±1.73%、37.8%±2.39%,凋亡指数分别为0.67±0.27、6.7±0.76.分别较对照组76.1%±6.54%、63.5%±3.28%、3.2±0.79之间的差异有统计学意义(P<0.05).结论 在ER阳性的乳腺癌细胞,BP1的表达受到ER传导通路的调节,E2可能通过调节BP1的表达水平而在乳腺癌的发生中起作用.BP1可能在调节细胞凋亡的过程中起到抑制作用.BP1阳性表达可能成为预后不良的标志. |
| 中文关键词:MCF-7细胞 BP1 细胞凋亡 原位杂交 bcl-2 免疫组化 |
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| Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line |
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| Abstract:Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10-8 mol/L) and tamoxifen (10-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma. |
| keywords:MCF-7 cell BP1 gene apoptosis in situ hybridization bcl-2 Immunohistochemistry |
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