| 离子对色谱法测定硫酸阿托品滴眼液的含量 |
| 投稿时间：2004-03-04 点此下载全文 |
| 引用本文：李志梅,刘萌,张黄丹.离子对色谱法测定硫酸阿托品滴眼液的含量[J].药学实践杂志,2004,(4):230~232 |
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| 中文摘要:目的 建立测定硫酸阿托品滴眼液中硫酸阿托品含量的方法。方法 采用Alhech-C18(5μm 4.6×250mm)色谱柱固定相,以乙腈-0.05mol/L庚烷磺酸钠水溶液(35:65)为流动相,检测波长210nm。结果 硫酸阿托品在8-120μg/mL浓度范围内线性良好,线性回归方程为:A=-72915.4073+15308.1639C,r=0.999,平均回收率为100.2%,RSD=0.65%。结论 本法操作简单,快速,准确可靠,回收率高。 |
| 中文关键词:硫酸阿托品 滴眼液 离子对色谱法 含量测定 |
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| Determination of atropine sulfate eye drops by ion-pair HPLC |
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| Abstract:Objective An ion-pair HPLC method was developed for determination of atropine sulfate in atropine sulfate eye drops. Methods Alltech-C18 (4.6×250mm, 5μm)column was used and the mobile phase was acetonitrile -0.05mol/L sodium hep-tanesul fonate (35:65). It was detected at 210nm. ResultsLinearity was obtained in the concentration rage of 8-120μg/mL, A=-72915.4073 + 15308.1639C,r=0.999. The average recoveries was 100.2%, RSD = 0.65%. Conclusion The method provide a accurate and sensitive way for the determination of atropine sulfate in atropine sulfate eye drops by ion-pair HPLC. |
| keywords:atropine sulfate eye drops ion-pair HPLC determination |
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