高效液相色谱法测定咽舒糖浆中绿原酸的含量

张广春; 赵昕; 王旭明; 陈军

高效液相色谱法测定咽舒糖浆中绿原酸的含量

投稿时间：2004-01-01 点此下载全文

引用本文：张广春,赵昕,王旭明,陈军.高效液相色谱法测定咽舒糖浆中绿原酸的含量[J].药学实践杂志,2004,(4):228~230

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作者	单位
张广春	中国人民解放军沈阳军区联勤部药品仪器检验所, 辽宁沈阳 110026
赵昕	中国人民解放军沈阳军区联勤部药品仪器检验所, 辽宁沈阳 110026
王旭明	中国人民解放军沈阳军区联勤部药品仪器检验所, 辽宁沈阳 110026
陈军	中国人民解放军沈阳军区联勤部药品仪器检验所, 辽宁沈阳 110026

中文摘要:目的采用高效液相色谱法,建立咽舒糖浆中绿原酸的含量测定方法。方法采用ODS分析色谱柱(4.6mm×250mm,7μm);流动相为乙腈-0.4%磷酸溶液(15:85);流速:1mL/min;检测波长:327nm。结果 HPLC法测定绿原酸可达基线分离,在1.62-21.60μg线性良好,回归方程:A=2516409C-2979,r=1.0000,5次测定平均加样回收率为100.1 %,RSD为0.80%。结论该法操作简便,结果准确,重现性好,可作为该制剂的质量控制方法。

中文关键词:咽舒糖浆高效液相色谱法绿原酸

Determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography

Abstract:Objective To develop a method for determination of chlorogenic acid in Yanshu Tangjiang by high performance liquid chromatography. Methods ODS C₁₈ (4.6mm×250mm, 7μm) was selected and the mobile phase consisted of acetonitrile -0.4% phosphoric acid (15:85)at the flow rate 1mL/min. The detection wavelength was 327nm. ResultsThe calibration curve was linear in the range of 1.62-21.60μg(r =1.0000). The average recovery of the method was 100.1%,RSD was 0.80% (n = 5). Conclusion This method was reliable and accurate. The method can be used to control the quality of preparation.

keywords:Yanshu Tangjiang HPLC chlorogenic acid

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