| HPLC法测定复方罗布麻片Ⅰ中盐酸异丙嗪和氯氮(艹卓)的含量 |
| 投稿时间：2003-10-28 点此下载全文 |
| 引用本文：李冰,胡德福,刘斐.HPLC法测定复方罗布麻片Ⅰ中盐酸异丙嗪和氯氮(艹卓)的含量[J].药学实践杂志,2003,(6):367~369 |
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| 中文摘要:目的 采用高效液相色谱法考察并建立了测定复方罗布麻片Ⅰ中盐酸异丙嗪和氯氮(艹卓)含量的方法。方法 色谱柱为Phenomenex-ODS3柱(250×4.60mm,5μm),甲醇-磷酸盐缓冲溶液(取磷酸二氢胺2.64g,加水1000mL溶解后,用磷酸调节pH值至3.0)(55:45)为流动相,流速1.0mL/min,检测波长为250nm,进样量20μL。结果 盐酸异丙嗪的线性范围为5.1-60.8μg/mL(r=1.0000),氯氮(艹卓)的线性范围为5.2-60.4μg/mL(r=0.9999),平均回收率分别为99.5%和101.2%。结论 本法精密度好,结果准确可靠,适用于该复方制剂的质量检验分析。 |
| 中文关键词:复方罗布麻片 盐酸异丙嗪 氯氮(艹卓) 高效液相色谱法 |
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| HPLC determination of promethazine hydrocloride and chlordiazepoxide in Fu-fang Luobuma Pian I |
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| Abstract:OBJECTIVE Promethazine Hydrocloride and Chlordiazepoxide were determined by HPLC in Fu-fang Luotuma Pian I. METHODS Phenomenex - ODS3 Column(250×4. 60mm,5μm),the mobile phase consisted of a mixtur mixture of methanol-phosphate buffer(dissolved 2.64g NH4H2P04 in 1000mL water adjusted pH to 3.0 with H3PO4)(55:45)at a flow rate of 1.0 mL/min,detection was done at 250nm,the injection volume was 20μL. RESULTS:The method offered good linearity 5.1-60.8μg/mL for promethazine hydrocloride(r=1.0000), and 5.2-60.4μg/mL for chlordiazepoxide(r=0.9999).The average recovery rates were 99.5% and 101.2% respectively. CONCLUSION This method is accurate and reliable for the quality control of this compound preparation. |
| keywords:Fufang Luobuma Pian I promethazine hydrocloride chlordiazepoxide HPLC |
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