| 青黛超临界提取物中靛蓝和靛玉红的含量测定研究 |
| 投稿时间：2002-06-10 点此下载全文 |
| 引用本文：姚文,王晓波,袭荣刚.青黛超临界提取物中靛蓝和靛玉红的含量测定研究[J].药学实践杂志,2002,(5):291~293 |
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| 中文摘要:目的 本文采用磺化-紫外分光光度法及一阶导数紫外分光光度法,对青黛药材及青黛超临界提取物进行含量测定。方法 样品经磺化反应后,用零阶光谱在 623nm波长处测定靛蓝的含量,用一阶导数紫外分光光度法在 482nm波长处测定靛玉红的含量。结果 靛蓝的线性范围为 1.14～5.32μg·ml-1,r=0.9999,平均回收率为 101.06%,RSD为 1.27%,靛玉红的线性范围为 0.52～5.2μg·ml-1,r=0.9999,平均回收率为 101.80%,RSD为 0.75%,放置 8h对含量测定无影响。结论 测定数批青黛药材及青黛超临界提取物样品,其靛蓝含量测定与药典法测定结果无显着差别。 |
| 中文关键词:靛蓝 靛玉红 紫外分光光度法 一阶导数紫外分光光度法 含量测定 |
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| Studies on the determination of indigotin and indirubin contents in Indigo Naturalis with Supercritical Fluid Extraction |
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| Abstract:OBJECTIVE To determine the contents of indigotin and indirubin in Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. METHODS The indirubin content was determined with sulfonate reaction-first order derivative-UV spectrophotometry; the indigotin content was determined with sulfonated reaotion-UV spectrophotometry.RESULTS The average recovery rate of indirubin was 101.80%,RSD=0.75%,and the average recovery rate of indigotin was 101.06%,RSD=1.27%.CONCLUSTION This method is simple and repeatable, and can be used as the quality control for method for the Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. |
| keywords:indigotin indirubin UV spectrophotometry first order derivative UV spectrophotometry contents determination |
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