| HPLC法测定诺氟沙星胶囊含量的改进 |
| 投稿时间：2001-09-28 点此下载全文 |
| 引用本文：李铭.HPLC法测定诺氟沙星胶囊含量的改进[J].药学实践杂志,2002,(2):97~99 |
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| 中文摘要:目的 改进诺氟沙星胶囊含量HPLC测定方法。方法 采用反相高效液相色谱法。色谱柱:SpherisorbC18柱,流动相:磷酸-三乙胺缓冲液:水:乙腈 (60:30:10),流速:1.0ml·min-1,检测波长:278nm.结果 诺氟沙星的浓度在 4.8～96μg·ml-1范围内线性关系良好,回归方程:A=2.19× 104+7.40×104C(r=0.99998),加样回收率平均值为 98.5%.RSD=0.5%,n=5).结论 用本方法测定诺氟沙星胶囊的含量快速、重现性好、专属性强、结果准确可靠。 |
| 中文关键词:诺氟沙星胶囊 高效液相色谱法 含量测定 |
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| Improvement of determination of norfloxacin capsules by HPLC |
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| Abstract:OBJECTIVE Norfloxacin capsules was determined by a improved HPLC method. METHODS The separation was performed on a spherisorb C18 column.The mobile phase was composed of phosphoric acid-triethylamin buf fer-water-acet onitrile(60:30:10).The eluate with a flow rate of 1.0ml·min-1 was detected at the wavelength of 278nm.RESULTS The calibration curves were good linear within the range of 4.8～96μg·ml-1(r=0.99998).The average recovery of norfloxacin was 98.5%,with RSD of 0.5%(n=5).CONCLUSION The improved method is rapid,good repeatabitity,accurate,reliable and suitable for determination of norfloxacin capsules. |
| keywords:Norfloxacin capsules HPLC determination of content |
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