| 腺苷预适应对缺血再灌注损伤的保护作用 |
| 投稿时间：2001-04-05 点此下载全文 |
| 引用本文：胡佳乐,李伟,沈甫明.腺苷预适应对缺血再灌注损伤的保护作用[J].药学实践杂志,2001,(4):206~208,205 |
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| 中文摘要:目的 观察预适应(缺血及腺苷)对大鼠下肢缺血再灌注后心、肺损伤的保护作用。方法 雄性SD大鼠随机分4组,每组8只。行腹主动脉下端阻断及再灌注各120min。组Ⅰ为手术对照组。组Ⅱ在缺血再灌注前先行阻断腹主动脉5min后开放5min,共4次。组Ⅲ麻醉后经颈静脉持续滴注腺苷生理盐水溶液。组Ⅳ为假手术对照组。 4组均于麻醉后经舌静脉注入伊文氏兰(Evan'sbluedye)溶液,动脉插管测定再灌注后的平均动脉压(MAP)。实验终点取心、肺组织行病理检查,心肌ATP酶,心、肺组织丙二醛(malonyldiade hyde,MDA)含量及Evan氏兰含量测定。 结果 组Ⅰ、Ⅲ再灌注后MAP均显着低于组Ⅱ、组Ⅳ(P>0.05),组Ⅰ、Ⅲ间,组Ⅱ、Ⅳ间无显着差异。组Ⅰ心肌ATP酶(包括Na+K+ATPase,Mg2+ATPase及Ca2+ATPase)活力均低于余3组,余3组间2,2比较均无显着差异。组Ⅰ心,肺组织MDA含量显着高于余3组,余3组间 2,2比较均无显着差异。组Ⅰ肺Evan氏兰含量显着高于Ⅱ、Ⅲ、Ⅳ组,组Ⅱ、Ⅲ高于组Ⅳ,组Ⅱ、Ⅲ间无显着差异。结论 缺血和腺苷预适应能改善大鼠下肢缺血再灌注后心,肺组织和功能损伤。 |
| 中文关键词:腺苷 预适应 缺血再灌注损伤 |
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| The protective effect of adenosine preconditioning on ischaemia-reperfusion injury |
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| Abstract:OBJECTIVE To investigate the protective effects of preconditioning on cardiac and pulmonary injury induced by lower extremity ischaemia-reperfusion in rats METHODS Animals were randomized into 4 groups and underwent laparotomy The abdominal aorta was isolated and clamped below the renal artery level for 120min then declamped for another 120min Group Ⅰ(n=8)was taken as control Group Ⅱ(n=8) was pretreatedwith ischaemia preconditioning that the lower extremities had experienced 4 times of ischaemia and reperfusion 5 min each before the 240 min treatment began Group Ⅲ(n=8) accepted continuous drip infusion of adenosine Group Ⅳ(n=8)was sham operated All groups were given Evan's blue dye intravenously after anesthesia Mean artery pressure(MAP), myocardium ATPase activities and malonyldiadehyde(MDA), pulmonary MDA and Evan's blue were determined separately. RESULTS MAP was lower in groupⅠand Ⅲ than in Ⅱ and Ⅳ,neither group Ⅰand Ⅲ, norⅡand Ⅳ, had significant differences(Ⅰ:61±14;Ⅱ:86±12; Ⅲ:69±18;Ⅳ:94±23mmHg) Myocardium ATPase activity was lower in group Ⅰthan in group Ⅱ,Ⅲ,Ⅳ(Na+ K+ ATPase 8.1±0.4 vs 8.9±2.1, 9.4±1.0and 9.0±0.8U/mg; Ca2+ ATPase 5.0±0.2 vs 5.3±1.0, 5.9±0.7and 5 7±0 8U/mg; Mg2+ ATPase 4.4±0.3 vs 5.2±0.8, 5.1±0.71and 5.3±0.6 U/mg),the MDA level was higher in groupⅠthan in group Ⅱ,Ⅲ,Ⅳ(MDA 2.5±0.4 vs 1.8±0.7, 1.6±0.5 and 1.7±0.7nmol/mg) Pulmonary MDA and Evan's blue level were higher in group Ⅰthan in the other three groups(MDA 2.3±0.2vs 1.6±0.4, 1.8±0.5 and 1.4±0.6nmol/mg; Evan's blue 3.1±1.2 vs 1.6±0.5, 1.5±0.3and 0.9±0.2μg/mg) CONCLUSION Ischaemia and adenosine preconditioning have some protective effects on cardiac and pulmonary injury induced by ischaemia reperfusion of lower extremity in rats. |
| keywords:adenosine preconditioning ischaemia-reperfusion injury |
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