| 不同产地红曲基原菌的RAPD分析 |
| 投稿时间：2001-02-27 点此下载全文 |
| 引用本文：邢旺兴,宓鹤鸣,陈士景,贺祥,吴玉田.不同产地红曲基原菌的RAPD分析[J].药学实践杂志,2001,(3):163~165,153 |
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| 基金项目:国家中医药管理局重点课题基金资助项目(97Z027) |
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| 中文摘要:目的 建立中药红曲基原菌的RAPD分析方法。方法 CTAB法提取不同产地红曲基原真菌的基因组DNA;琼脂糖凝胶电泳法检测PCR扩增产物,PHYLIP3.5c进行聚类分析。结果 S282等10个随机引物的扩增产物谱带多,特征好;产地间指纹图谱呈现出明显的DNA扩增产物多态性。结论 RAPD分析技术可作为红曲鉴定的有用手段。 |
| 中文关键词:红曲 RAPD 鉴别 |
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| Analysis of the origin fungi of the traditional Chinese medicine, Hongqu from different producing areas with RAPD technique |
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| Abstract:OBJECTIVE Establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the traditional Chinese medicine, Hongqu. METHODS The genome DNAs of the origin fungi of Hongqu from 14 different producing areas were extracted by CTAB. Amplification products were detected by agarose gel elect rophoresis and done clustering analysis using PHYLIP3.5c.RESULTS A characteristic pattern was produced dependent on one of the 10 screened random primers and the genome amplified.The diversities of the fingerprint patterns of Monascus exist obvious by which we can distinguish easily.CONCLUSION RAPD as-say technique is a useful tool to identify of Hongqu. |
| keywords:Hongqu RAPD identification |
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