| 关附甲素对培养心肌细胞"缺氧缺糖"的保护作用 |
| 投稿时间：2000-08-28 点此下载全文 |
| 引用本文：陈清,魏丕敬,顾培坤,金正钧,周中源.关附甲素对培养心肌细胞"缺氧缺糖"的保护作用[J].药学实践杂志,2000,(5):331~333 |
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| 中文摘要:目的 研究关附甲素对培养心肌细胞"缺氧缺糖"的保护作用。方法 以培养心肌细胞的"缺氧缺糖"模拟全体的缺血性损伤,以传统的钙离子拮抗剂---维拉帕未为对照药,观察关附甲素对"缺氧缺糖"心肌细胞乳酸脱氢酶(LDH)生成,脂质过氧化代谢产物丙二醛(MDA)的生成的影响。结果 关附甲素30ug/ml即能减少LDH的释放和MDA的生成(P<0.05),且和维拉帕未有一定的相似性。结论 关附甲素对"缺氧缺糖"心肌细胞具有一定的保护作用,且具有抗脂质过氧化作用,心肌细胞的保护作用可能和其抗脂质过氧化或抑制外钙内流有关。 |
| 中文关键词:关附甲素 乳酸脱氢酶 丙二醛 维拉帕米 |
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| Effects of Guan-fu base A on neonatal rat myocytes in cultures with oxygen and glucose deprivation |
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| Abstract:OBJECTIVE To investigate the effects of GFA on neonatal rat myocytes in cultures with oxygen and glucose deprivation(CMOGD).METHODS By taking cultured neonatal rat myocytes with oxygen and glucose deprivation as ischemia-mimic condition of body and the Ca2+-antagonist Verapamil as control,the leakage of lactic dehydrogenase(LDH) and the production of malondialdehyde(MDA) were investigated. RESULT GFA 30ug/ml suppessed the lactic dehydrogenase (LDH) leakage and the production of malondialdehyde (MDA) in CMOGD (P<0.05). The effects on LDH and MDA were concentration-dependant. During the experiment GFA was compared with Verapamil, the traditional Ca--antagonist. It was found the GFA had similar function with Verapamil. CONCLUSION GFA has a cytoprotection on CMOGD,Possibaly acts as an antiperoxidant and/or inhibiter of Ca2+ influx. |
| keywords:guan-fu base A lactic dehydrogenase(LDH) malondialdehyde(MDA) Verapamil. |
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