| 反相高效液相色谱法测定对乙酰氨基酚血药浓度 |
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| 引用本文：陈坚,方维军,张灯玲,丁宗政,陈睿.反相高效液相色谱法测定对乙酰氨基酚血药浓度[J].药学实践杂志,1998,(5):296~298 |
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| 中文摘要:建立了反相高效液相色谱法测定对乙酰氨基酚血浆中浓度的方法。方法使用YWGC18柱(4.6×250mm,10μm)；流动相为甲醇：醋酸－醋酸钠：二乙胺(20：80：0.5)；以茶碱为内标物；血清处理用甲醇沉淀蛋白；紫外检测波长244nm；流动相流速1.5ml/min；最低检出浓度0.05μg/ml。对乙酰氨基酚血药浓度在0.25~25μg/ml范围内线性关系良好(r＝0.9999)。本方法适用于对乙酸氨基酚的血药浓度测定。 |
| 中文关键词:对乙酰氨基酚 反相高效液相色谱法 血药浓度 |
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| Determination of paracetamol in human serum by HPLC |
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| Abstract:An assay for paracetamol in human serum was developed using reverse phase high-performance liquid chromatographic with UV detection at 244nm. Analytial column of YWG C18 is used. The mobile phase consisted of a mixture of methanol: acetic acid-sodium acetate buffer solution: diethylamine (20:80:0.5)at the flow rate of 1.5ml/min. Theophylline is used as internal standard. Methanol is used for the sedimentation of protein. And minimum detective concentaion 0.05μg/ml. The standard curve is linear in the rang of 0.25~25μg/ml,the correlation coefficient is 0.9999.The method is suitable for the determination of human serum paracetamol and pharmacokinetic reserches. |
| keywords:paracetamol HPLC plasma concentration |
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