| 高效液相色谱间接光度检测法测定心脏停搏液中钠、钾、钙、镁的含量 |
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| 引用本文：袁成,王景祥,梁竹,焦素云,李凡东.高效液相色谱间接光度检测法测定心脏停搏液中钠、钾、钙、镁的含量[J].药学实践杂志,1997,(4):234~237 |
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| 中文摘要:本文用高效液相色谱间接光度检测法测定了心脏停搏液中钠、钾、钙、镁的含量。以Soherisorb SCX为固定相,0.35mmol/L硫酸铈的[水-乙腈(85:15),并用磷酸调pH至3.2～3.4]溶液为流动相,亚铈离子作为流动相平衡离子直接应用光度检测器测定,并与其他测定方法比较。结果表明,分离度较好,104%>回收率>97%,线性关系良好。结果与滴定法相比无显著性差异。该法具有操作简便快速,灵敏准确,适于作为制剂中4种离子的常规检测方法。 |
| 中文关键词:心脏停搏液 钠 钾 钙 镁 HPLC-IPD |
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| Determination of sodium, kalium, magesium and calcium in liquor of cardioplegia by high performance liquor chromatography-indirect photothetric determination method |
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| Abstract:To determine the content of sodium,kalium, magnesium and calcium in liquor of cardioplegia by HPLC-IPD method.METHOD: The chromatographic system consisted of Soherisorb SCX column and mobile phase of the solution of 0.35 mmol/L cerium sulphate in water-acetonitrile(85:15), that were regulated to pH 3.2～3.4 with phosphoric acid.Ceritnn was a balance-ion in mobile phase, therefore the content of sodium, kalium, magnesium and calcium were determined directly by photometer.The detected wave length was UV254 um.RESULTS:The mean recovery of 4 ion were between in 97～104% and deteCtion limits of sodium,kalium,magnesium and calcium were 5, 8, 5 and 4 ng.There was noremarkable betWeen in this HPLC-IPD method and titrative method.CONCLUSION: The method is constant, sensitive, have a concentration and is good for determination of sodium, kalium, magnesium and calcium concentration. |
| keywords:liquor of cardioplegia sodium kalium magnesium calcium HPLC-IPD |
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