| 高效液相色谱法测定尿中咖啡因主要代谢物浓度 |
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| 引用本文：易涛,卢建丰.高效液相色谱法测定尿中咖啡因主要代谢物浓度[J].药学实践杂志,1997,(2):94~99 |
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| 中文摘要:本文建立了测定尿中咖啡因5种主要代谢物(AFMU、1X、1U、17X、17U)浓度的高效波相色谱法。尿样用硫酸铵饱和后加氯仿及异丙醇混合液(85:15)提取2次,空气流吹干,蒸馏水溶解进样。以Shim PackC18为固定相,甲醇-动腈-0.05%醋酸(10:1:89)为流动相,扑热息痛为内标,流速为1.2ml/min,在280um处定量检测。本法精确稳定可靠。用此方法测定了120名健康志愿者口服咖啡后的尿样,对人体内N-乙酰化转移酶和CYP1A2酶活性作了初步分析。 |
| 中文关键词:咖啡因 HPLC N-乙酰化转移酶 CYP1A2酶 |
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| HPLC determination of caffeine metabolites in urine |
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| Abstract:A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U).The samplb added ammonium sulfate was extracted twice by a mixture of chloroform-isopropanol(85:15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃.HPLC analysis was carried out on Shim Pack C18 column with methanol-ace-tonitrile-0.05% acetic acid (10:1:89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caffeine in a healthy sample(n = 120).Caffeine metabolite ratios were used to assess the poymorphicN-acetyltransferase and CYP1A2 activities. |
| keywords:Caffeine HPLC N-acetyltransferase CYP1A2 |
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