| 高效液相色谱法测定葛根总黄酮及制剂中葛根素的含量 |
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| 引用本文：郭建平,孙其荣,周全.高效液相色谱法测定葛根总黄酮及制剂中葛根素的含量[J].药学实践杂志,1996,(5):300~301,299 |
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| 中文摘要:以反相高效液相色谱法(RP-RPLC)测定葛根总黄酮提取物及制剂中葛根素的含量。检测波长250nm,流动相为甲醇-水(25:75),进样20μl采用外标法。当葛根素浓度在0.5-12μg/ml时,浓度与峰面积呈良好线性关系,r=0.9991.回收率及RSD分别为100.9%,1.28%. |
| 中文关键词:反相高效液相色谱法 葛根 葛根素 愈风宁心片 葛根总黄酮 葛根黄酮控释胶囊 |
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| RP-HPLC method for the determination of puerarin in isoflowonesd from Radix puerariae and its preparations |
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| Abstract:A RP-HPLC method for the determination of Puerarin in isoflowonesd from Radix Puerariae and its preparations and a C 18 column and a mobile phase of methanol-water (25:75) were employed.Puerarin was determined at 250nm.The recovery rate of the Puerarin was 100.9%.The rigression equation was X=466.83+8501.25C(r=0.9991),The method is simple,sensitive and accurate. |
| keywords:RP-HPLC puerarin |
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