| RP－HPLC测定葛根芩连片中葛根素的含量 |
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| 引用本文：谭生建,邱夏,李光慧,赵炳成,梁玉琴.RP－HPLC测定葛根芩连片中葛根素的含量[J].药学实践杂志,1996,(5):295~297 |
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| 中文摘要:本文报道RP-HPLC测定葛根芩连片中葛根素含量。采用Shim-PackCLC-ODS(15cm×6mmID)分析柱;北分厂ODS保护柱;流动相:甲醇-水(23:77);流速:1.0ml/min;色谱柱温度:35℃;检测波长:250nm;进样体积10μl;外标法计算含量。理论板数按葛根素峰计算不低于2000;葛根素浓度在5μg/ml-80μg/ml范围与色谱峰面积呈线性关系;R=0.9999;回收率及RSD分别是97.6%,1.8%。 |
| 中文关键词:RP－HPLC 葛根芩连片 葛根素 |
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| Determination of puerarin in Gegen qinlian pian by RP-HPLC |
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| Abstract:A quantitative method was developed for the determination of puerarin in Gegen Qing lian Pian by reversed phase HPLC.Chromatographic conditions included column ODS-C(10),column temperature,35℃;detector UV,250nm;mobile phase,methanol-water(23:77),flow rate 1 ml/min;injecting volume,10μl;external standad.the number of theoretical plates calculated by puerarin peak was no less than 2000.The standard curve was linear in the concentration range of 5μg/ml-80μg/ml,and correlation coefficient was 0.9999.The average rccovery and the relative standsrd deviation were 97.6% and 1.8%,respectively. |
| keywords:RP-HPLC Gegen Qinglian Pian Puerarin |
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