| 联用超高效液相色谱-高分辨飞行时间质谱技术对扶正化瘀胶囊化学成分快速鉴别 |
| 投稿时间：2019-01-09 修订日期：2019-03-29 点此下载全文 |
| 引用本文：陶朝阳,邢心睿,陈思,曹奇,丁德英,朱臻宇.联用超高效液相色谱-高分辨飞行时间质谱技术对扶正化瘀胶囊化学成分快速鉴别[J].药学实践杂志,2019,37(5):406~415 |
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| 基金项目:国家自然科学基金（81773683） |
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| 中文摘要:目的 运用超高效液相色谱-高分辨飞行时间质谱（UHPLC-TOF/MS）联用技术鉴别扶正化瘀胶囊中化学成分。方法 色谱分离采用ACQUITY UPLCHSS T3柱（2.1 mm×100 mm，1.8 μm）；流动相0.1%甲酸水溶液（A）-0.1%甲酸乙腈溶液（B），梯度洗脱条件：0~3 min，2% B；3~18 min，2%~50% B；18~22 min，50%~95% B；22~25 min，95% B。平衡时间为10 min，流速为0.4 ml/min，分析时间为25 min。质谱定性采用电喷雾离子源，正/负离子模式扫描，采集范围100~1 100 m/z。结果 正/负离子模式下，一次性鉴别出154个成分，其中，正、负离子模式下均有响应的有28个。结论 较全面地阐明了扶正化瘀胶囊的成分组成，为该复方的深入研究奠定了基础。 |
| 中文关键词:扶正化瘀胶囊 鉴别 化学成分 |
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| Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS |
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| Abstract:Objective To identify the chemical constituents in Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time of flight mass spectrometry(UHPLC-TOF/MS).Methods ACQUITY UPLCHSS T3(2.1mm×100mm,1.8μm) was used to chromatographic separation; mobile phase 0.1% formic acid aqueous solution(A) -0.1% formic acid acetonitrile solution(B),gradient elution conditions:0-3min,2% B; 3-18 min,2%-50% B; 18-22 min,50%-95% B; 22-25 min,95% B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min.Mass spectrometry was performed by an electrospray ion source using a positive-negative ion mode scan with a range of 100-1 100 m/z.Results 154 components were identified at one time,of which 28 were positive and negative ion modes.Conclusion The composition of Fuzheng Huayu capsule was comprehensively clarified in this study,which laid a foundation for further research of this compound. |
| keywords:Fuzheng Huayu capsule identification chemical composition |
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