| 双波长HPLC法测定止痒地霜中地塞米松和羟苯乙酯的含量 |
| 投稿时间：2019-02-26 修订日期：2019-06-10 点此下载全文 |
| 引用本文：郝哲,邱会苹,王丽军,刘栋,宋艳丽,王伟勤,屈俊.双波长HPLC法测定止痒地霜中地塞米松和羟苯乙酯的含量[J].药学实践杂志,2019,37(4):361~364 |
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| 中文摘要:目的 建立双波长HPLC法同时测定止痒地霜中地塞米松磷酸钠和羟苯乙酯的含量。方法 采用RP-HPLC法，以Wondasil C18（4.6 mm×250 mm，5 μm）为色谱柱，以三乙胺溶液-甲醇-乙腈（52：43：5）为流动相；检测波长为242 nm，流速为1.0 ml/min。结果 羟苯乙酯和地塞米松磷酸酯分别在21.42～64.26 μg/ml （r=1.0000）和20.84～62.53 μg/ml （r=0.9999）范围内有良好的线性关系，平均回收率分别为99.1%（RSD=0.41%）和99.0%（RSD=0.72%）。结论 该法简便、准确、重复性好，可用于止痒地霜的质量控制。 |
| 中文关键词:止痒地霜 地塞米松磷酸钠 羟苯乙酯 |
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| Determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream by HPLC of double wavelength |
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| Abstract:Objective To establish a double wavelength HPLC method for determination of dexamethasone sodium phosphate and ethylparaben in Zhiyang dishuang cream.Methods The HPLC method was adopted with the Wondasil C18(4.6 mm×250 mm,5 μm) and triethylamine solution-methanol-acetonitrile (52:43:5) as the mobile phase at the rate of 1 ml/min.The detection wavelength was 242 nm.Results The linear ranges of ethylparaben and dexamethasone phosphate were 21.42-64.26 μg/ml(r=1.0000)and 20.84-62.53μg/ml(r=0.9999)respectively.The average recoveries of the three contents were 99.1% and 99.0%,while RSD were 0.41% and 0.72%.Conclusion The method is simple,accurate,reproducible and suitable for quality control of Zhiyang dishuang cream. |
| keywords:Zhiyang dishuang cream dexamethasone sodium phosphate ethylparaben |
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