| 高效液相色谱法测定不同产地假蒟中的α-细辛脑含量 |
| 投稿时间：2018-09-27 修订日期：2019-03-07 点此下载全文 |
| 引用本文：李清,何明月,瞿发林,谭兴起,董文燊,倪玉佳,邵留英.高效液相色谱法测定不同产地假蒟中的α-细辛脑含量[J].药学实践杂志,2019,37(4):348~351 |
| 摘要点击次数: 1308 |
| 全文下载次数: 885 |
|
| 基金项目:原南京军区重大专项课题（15DX005） |
|
| 中文摘要:目的 采用高效液相色谱（HPLC）法测定不同产地假蒟中α-细辛脑的含量。方法 色谱柱为Lichrospher C18柱（150 mm×4.6 mm，5 μm），流动相为乙腈-0.05%磷酸溶液（47：53），流速为1.0 ml/min，检测波长313 nm，柱温30℃。结果 α-细辛脑在0.0970~1.934 μg范围内与峰面积有良好线性关系（r=0.999 8），平均回收率为99.94%（RSD=1.13%），样品在24 h内稳定，且该方法重复性较好。结论 本方法简便准确，可用于假蒟药材中α-细辛脑的含量测定，以控制其质量；我国不同产地的假蒟中α-细辛脑含量有明显差异，其中以广西产假蒟含量最高。 |
| 中文关键词:假蒟 α-细辛脑 高效液相色谱 含量测定 |
| |
| Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC |
|
|
| Abstract:Objective To determine the content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC.Methods The chromatographic column was Lichrospher C18 (150 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.05% H3PO4 solution (47:53) with a flow rate of 1.0 ml·min-1,the UV detection wavelength at 313 nm and the column temperature at 30℃.Results There was a good linear relationship for α-Asarone in the range of 0.097 0~1.934 μg (r=0.999 8).The average recovery was 99.94% (RSD=1.13%).The sample was stable within 24 h.The method had good repeatability.Conclusions This method is simple and accurate for the content assay of α-Asarone in Piper sarmentosum Roxb.It can be used to control the quality of Piper sarmentosum Roxb.There was a significant difference of α-Asarone content in Piper sarmentosum Roxb.from different provinces in China.The Piper sarmentosum Roxb.from Guangxi province has the highest content of α-Asarone. |
| keywords:Piper sarmentosum Roxb. α-Asarone HPLC content determination |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
