| 高效液相色谱法测定肝安胶囊中水飞蓟宾的含量 |
| 投稿时间：2012-09-26 修订日期：2013-03-26 点此下载全文 |
| 引用本文：王慧,路克军,张洁瑾,栾晓琳,周燕妮,张国庆.高效液相色谱法测定肝安胶囊中水飞蓟宾的含量[J].药学实践杂志,2013,31(6):459~461 |
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| 中文摘要:目的 建立高效液相色谱法测定肝安胶囊中水飞蓟宾的含量。方法 使用Agilent Zorbax SB-C18柱(4.6 mm×250 mm,5 μm),流动相:1%冰醋酸-甲醇=50:50,流速:1.0 ml/min,柱温:30℃,检测波长:287 nm,测定10批肝安胶囊中水飞蓟宾的含量。结果 水飞蓟宾在10.28~123.36 μg/ml(r=1)浓度范围内线性关系良好,回收率为97.00%(RSD=0.68%)。10批肝安胶囊中水飞蓟宾含量为11.11~16.65 mg/粒。结论 该方法简便、准确、重复性好,可以用于肝安胶囊的质量控制。 |
| 中文关键词:高效液相色谱法 水飞蓟宾 肝安胶囊 含量测定 |
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| Determination of silybin contents in Gan'an capsule by HPLC |
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| Abstract:Objective To establish a HPLC method for determination of silybin contents in Gan'an capsule. Methods Angilent Zorbax SB C18(4.6 mm×250 mm, 5 μm) column was used with 1% acetic acid:methanol=50:50 as the mobile phase. The flow rate was 1.0 ml/min, and the temperature was at 30℃.The detection wavelength was 287 nm.Determination of silybin contents in Gan'an capsule of 10 batches of preparations.Results The silybin calibration curve was at the range of 10.28~123.36 μg/ml(r=1)with average recovery of 97.00%(RSD=0.68%).10 batches of preparations silybin contents in Gan'an capsule was 11.11~16.65 mg/granule.Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan'an capsule. |
| keywords:HPLC silybin Gan'an capsule determination |
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